{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:31:05Z","timestamp":1772897465763,"version":"3.50.1"},"reference-count":1,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2001,4,23]],"date-time":"2001-04-23T00:00:00Z","timestamp":987984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,4,23]],"date-time":"2001-04-23T00:00:00Z","timestamp":987984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<jats:p>Proximal tubule cells (PTC) in chronic renal disease produce chemokines which cause renal interstitial inflammation, and also transport more Na&lt;sup&gt;+&lt;\/sup&gt; than normal. To investigate whether these two events might be related, monocyte chemoattractant protein-1 (MCP-1) production was examined in rat PTC in primary culture. Amiloride reduced, while ouabain increased levels of MCP-1 mRNA and protein. Amiloride reduced MCP-1 in cells stimulated with ouabain, lipopolysaccharide (LPS) or albumin. Intracellular Na&lt;sup&gt;+&lt;\/sup&gt; rose with ouabain, but not LPS or albumin. Effects of amiloride, ouabain, LPS and albumin were abrogated by sodium-free but not chloride-free culture medium, and were not explained by changes in intracellular pH. Intracellular Ca&lt;sup&gt;2+&lt;\/sup&gt; rose with ouabain, LPS or albumin and sodium-free medium. BAPTA-AM reduced intracellular Ca&lt;sup&gt;2+&lt;\/sup&gt; and MCP-1 mRNA levels in unstimulated cells, and cells stimulated with ouabain, LPS or albumin. Thus, amiloride and ouabain may alter tubular cell MCP-1 by changing intracellular Na&lt;sup&gt;+&lt;\/sup&gt;, with secondary changes in intracellular Ca&lt;sup&gt;2+&lt;\/sup&gt;, whereas stimulation by LPS and albumin may involve Ca&lt;sup&gt;2+&lt;\/sup&gt; directly.<\/jats:p>","DOI":"10.1159\/000052613","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:15Z","timestamp":1050433575000},"page":"205-213","source":"Crossref","is-referenced-by-count":5,"title":["Regulation of Tubular Cell MCP-1 Production by Intracellular Ions: A Role for Sodium and Calcium"],"prefix":"10.1159","volume":"9","author":[{"given":"Davis C.H.","family":"Harris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,4,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00905.x"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/52613","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/52613","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:15:17Z","timestamp":1745468117000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000052613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,23]]},"references-count":1,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2001,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000052613","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"value":"1660-2129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,23]]}}}