{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:49:03Z","timestamp":1761511743264,"version":"3.40.4"},"reference-count":3,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2001,4,23]],"date-time":"2001-04-23T00:00:00Z","timestamp":987984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,4,23]],"date-time":"2001-04-23T00:00:00Z","timestamp":987984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"Beside its role in calcium homeostasis, 1,25-D<sub>3<\/sub> modulates multiple immunological functions in cells of the immune system. In tubular epithelial cells, it increases the expression of HLA-DR and ICAM-1 molecules. Since production of chemokines, such as IL-8 and MCP-1, by tubular epithelial cells is crucial for the inflammatory response in acute transplant rejection and interstitial nephritis, we tested whether 1,25-D<sub>3<\/sub> influences the production of IL-8 and MCP-1 by primary human tubular epithelial cells (TEC). For chemokine detection we used enzyme-linked immunosorbent assays. We differentiated between chemokine secretion directed to the apical and basolateral environment by using cell culture inserts as a model for the tubular basement membrane. mRNA of IL-8 and MCP-1 after stimulation of TEC with IL-1\u03b1 and\/or 1,25-D<sub>3<\/sub> was isolated and compared by competitive RT-PCR. We found that basolateral production of IL-8 was higher than luminal secretion. 1,25-D<sub>3<\/sub> (10<sup>\u20138<\/sup><i>M<\/i>) alone and in combination with IL-1\u03b1 suppressed IL-8 production after 48 h. Basolateral compared to luminal MCP-1 secretion was higher after stimulation either with IL-1\u03b1 alone or combined with 1,25-D<sub>3<\/sub>. After 72 h, 1,25-D<sub>3<\/sub> enhanced the IL-1\u03b1-stimulated MCP-1 secretion. Increased IL-8 mRNA expression after stimulation with IL-1\u03b1 was suppressed by coincubation with 1,25-D<sub>3<\/sub>, while MCP-1 mRNA synthesis was enhanced by 1,25-D<sub>3<\/sub> alone and in combination with IL-1 \u03b1 . We conclude that 1,25-D<sub>3<\/sub> differently modulates the expression of CXC-chemokine IL-8 and CC-chemokine MCP-1 by human TEC. The differential effects of 1,25-D<sub>3<\/sub> on renal tubular cytokine secretion have to be considered in therapeutic dials on this hormone, e.g. in renal transplant rejection.<\/jats:p>","DOI":"10.1159\/000052615","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:15Z","timestamp":1050433575000},"page":"223-228","source":"Crossref","is-referenced-by-count":18,"title":["1,25-Dihydroxyvitamin D<sub>3<\/sub> Differentially Regulates IL-1\u03b1-Stimulated IL-8 and MCP-1 mRNA Expression and Chemokine Secretion by Human Primary Proximal Tubular Epithelial Cells"],"prefix":"10.1159","volume":"9","author":[{"given":"S.","family":"Kr\u00fcger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Kreft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,4,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1086\/314700"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000025914"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0966-3274(93)90062-D"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/52615","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/52615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:15:18Z","timestamp":1745468118000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000052615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,23]]},"references-count":3,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2001,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000052615","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"type":"electronic","value":"1660-2129"}],"subject":[],"published":{"date-parts":[[2001,4,23]]}}}