{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:51:53Z","timestamp":1773658313021,"version":"3.50.1"},"reference-count":1,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2000]]},"abstract":"<i>Background:<\/i> An association between allergy to <i>Ficus benjamina<\/i> and natural rubber latex (NRL) has been suspected based on clinical and immunological observations. The responsible cross-reactive allergens have not been identified yet. This study was undertaken to investigate the cross-reactivity between hevein (Hev b 6.02, 4.7 kD), a major allergen of NRL, and <i>F. benjamina<\/i> and identify its counterpart in <i>F. benjamina<\/i>. <i>Methods:<\/i> 89 serum samples from subjects allergic to NRL were used in the study. Skin prick tests were performed with highly purified hevein and sap extract of <i>F. benjamina<\/i>. Specific IgE antibodies to NRL, <i>F. benjamina<\/i> and Hev b 6.02 were determined by the Pharmacia CAP method. Cross-reactivity among these allergens was investigated by means of CAP and immunoblot inhibition experiments. Two-dimensional gel electrophoresis separation and protein microsequencing were performed to identify the cross-reactive allergens in <i>F. benjamina<\/i>. <i>Results:<\/i> 67 out of 89 (75%) sera showed elevated IgE to hevein. Specific IgE to <i>Ficus<\/i> were found in 22 (24.7%) sera, and with 1 exception, all these sera also had IgE to Hev b\u00a06.02. Results of CAP inhibition assays using 11 sera showing IgE to both Hev b 6.02 and <i>Ficus<\/i> demonstrated that the IgE to <i>Ficus<\/i> could be completely inhibited by Hev b 6.02 in 6 of 11 sera. Immunoblots and immunoblot inhibition assays revealed that a protein of about 45 kD in <i>F. benjamina<\/i> is strongly recognized by serum IgE. In addition, the IgE-binding reactivity to this 45-kD protein could be completely inhibited by preincubation of the sera with Hev b 6.02. N-terminal protein sequencing of 14 amino acids indicated that this 45-kD protein has a hevein-like domain at the N-terminal region and may belong to the endochitinase family. <i>Conclusion:<\/i> Latex-allergic<i><\/i>patients are at higher risk of becoming sensitized to <i>Ficus<\/i>. Hev b 6.02 in latex is a major cross-reactive allergen and its counterpart in <i>F. benjamina<\/i> is an acidic protein with a molecular weight of about 45 kD and a hevein-like N-terminal domain.<\/jats:p>","DOI":"10.1159\/000053641","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:15Z","timestamp":1050433575000},"page":"291-298","source":"Crossref","is-referenced-by-count":20,"title":["Identification and Characterization of Cross-Reactive Natural Rubber Latex and <i>Ficus benjamina <\/i>Allergens"],"prefix":"10.1159","volume":"123","author":[{"given":"Zhiping","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"D\u00fcser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Flagge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke","family":"Maryska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Sander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Raulf-Heimsoth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xaver","family":"Baur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,12,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.41.25394"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/53641","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/53641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:25:42Z","timestamp":1745468742000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000053641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":1,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2000,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000053641","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000]]}}}