{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:13:37Z","timestamp":1745554417778,"version":"3.40.4"},"reference-count":10,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2001]]},"abstract":"Disruption of the APC\/\u03b2-catenin\/Tcf pathway has been proposed as an important step in the development of cancer. The <i>Tcf-4<\/i> transcription factor gene was reported to be one of the targets of microsatellite instability (MSI) in colorectal cancers in with MSI. We carried out a sequencing analysis of the <i>Tcf-4<\/i> gene in 41 Japanese patients with gastrointestinal tumors with MSI as well as in cancer cell lines. Three of 21 (14.3%) colorectal tumors with MSI contained a mutant <i>Tcf-4<\/i> gene encoding 1-bp deletion in an (A)9 repeat, leading to carboxyl terminal truncation of Tcf-4 protein. No <i>Tcf-4<\/i> mutations were detected in 20 gastric tumors with MSI, or in gastric cancer cell lines. Additionally, we found a novel transcript of the <i>Tcf-4<\/i> gene which contained a segment of 73 bp in front of the (A)9 repeat of the <i>Tcf-4<\/i> coding sequence. Sequencing analysis revealed that the inserted fragment was 60% homologous to that of exon IXA of the <i>Tcf-1<\/i> gene. It is of interest that this insertion resulted in truncation of Tcf-4, similar to the 1-bp deletion in the (A)9 repeat. Therefore, in part of the Japanese colorectal tumors with MSI, frameshift mutations in <i>Tcf-4<\/i> may be of functional significance. Functional alterations in the Tcf-4 signaling network in gastrointestinal tumorigenesis require further investigations.<\/jats:p>","DOI":"10.1159\/000055367","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:20Z","timestamp":1050433580000},"page":"156-161","source":"Crossref","is-referenced-by-count":8,"title":["Genetic Alterations in the Human <i>Tcf-4<\/i> Gene in Japanese Patients with Sporadic Gastrointestinal Cancers with Microsatellite Instability"],"prefix":"10.1159","volume":"61","author":[{"given":"Hiroshi","family":"Saeki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriko","family":"Tokunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Kawaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Maehara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keizo","family":"Sugimachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,8,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.17.10164"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5307.1784"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5307.1787"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5307.1790"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/18884"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5382.1509"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/ng1196-255"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5302.967"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/379499a0"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990701)86:1<58::AID-CNCR10>3.0.CO;2-S"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/55367","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/55367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:37:34Z","timestamp":1745469454000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000055367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":10,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2001,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000055367","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"type":"print","value":"0030-2414"},{"type":"electronic","value":"1423-0232"}],"subject":[],"published":{"date-parts":[[2001]]}}}