{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T05:10:20Z","timestamp":1745471420315,"version":"3.40.4"},"reference-count":11,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2001]]},"abstract":"The synuclein family of proteins is a group of primarily brain-expressed polypeptides that show a high degree of amino acid conservation. \u03b1-Synuclein is the best known of the synuclein family, as it is a major component of the Lewy body, a cytoplasmic inclusion characteristic of Parkinson\u2019s disease as well as a variety of related neurodegenerative disorders. With the discovery that mutations in \u03b1-synuclein can cause Parkinson\u2019s disease, a potential role for the other synuclein family members in neurodegenerative disease is being considered. \u03b2-Synuclein in particular may deserve special attention, as it is co-expressed with \u03b1-synuclein at presynaptic nerve terminals, is subject to phosphorylation by Ca<sup>2+<\/sup> calmodulin protein kinase II, appears important for neural plasticity, and forms aggregates in the brains of patients with Parkinson\u2019s disease and a related disorder. To facilitate study of \u03b2-synuclein, we have cloned the mouse \u03b2-synuclein gene (<i>Sncb<\/i>) and determined its genomic organization, size, and intron-exon structure. Using an interspecific backcross mapping panel from The Jackson Laboratory, we were then able to localize <i>Sncb<\/i> to chromosome 13 at the MGD 35.0 cM position. Like the human \u03b2-synuclein gene, <i>Sncb<\/i> appears to consist of six exons separated by five introns. Unlike the human \u03b2-synuclein gene, the mouse ortholog possesses a variant GC 5\u2032 splice donor sequence at the exon 4 \u2013 intron 4 boundary in a highly conserved splice junction consensus. Northern blot analysis and Western blot analysis both indicate that <i>Sncb<\/i> is highly expressed in the brain. Knowledge of the genomic organization and expression pattern of <i>Sncb<\/i> will allow functional studies of its potential role in neurodegeneration to commence in the mouse.\u00a0\u00a0\u00a0<\/jats:p>","DOI":"10.1159\/000056962","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:20Z","timestamp":1050433580000},"page":"117-123","source":"Crossref","is-referenced-by-count":6,"title":["Genomic organization, chromosome location, and expression analysis of mouse \u03b2-synuclein, acandidate for involvement in neurodegeneration"],"prefix":"10.1159","volume":"93","author":[{"given":"B.L.","family":"Sopher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.L.","family":"Koszdin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.E.","family":"McClain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.B.","family":"Myrick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.A.","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.C.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.R.","family":"La Spada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,7,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6408(1997)20:4<320::AID-DVG3>3.0.CO;2-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13450"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90040-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(20000215)59:4<528::AID-JNR8>3.0.CO;2-0"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00395-5"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/ng0298-106"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199810083391506"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5556"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5492.721"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5290.1197"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5321.2045"}],"container-title":["Cytogenetic and Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/56962","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/56962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:53:54Z","timestamp":1745470434000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000056962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":11,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2001,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000056962","archive":["Portico"],"relation":{},"ISSN":["1424-8581","1424-859X"],"issn-type":[{"type":"print","value":"1424-8581"},{"type":"electronic","value":"1424-859X"}],"subject":[],"published":{"date-parts":[[2001]]}}}