{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T05:38:02Z","timestamp":1775367482204,"version":"3.50.1"},"reference-count":7,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>B cell lymphoma develops in the pleural cavity of patients affected by long-standing pyothorax resulting from lung tuberculosis, thus termed pyothorax-associated lymphoma (PAL). PAL usually shows a diffuse large cell morphology, and constantly contains Epstein-Barr virus (EBV) genome. To investigate whether PAL cells proliferate in response to specific antigenic stimuli and its stage in B cell differentiation, immunoglobulin heavy chain gene in 7 cases and 2 cell lines from PAL, all confirmed by histological studies to be EBV-positive diffuse large B cell lymphoma, were examined by using polymerase chain reaction (PCR) method. Clonal rearrangement of the gene was detected in 4 cases of PAL tissues and one cell line. As for the usage of the V region gene (V&lt;sub&gt;H&lt;\/sub&gt;), the V&lt;sub&gt;H&lt;\/sub&gt;3 family gene was used in 3 of these 5 cases with different homologous germlines, suggesting that the origin of PAL cells from a repertoire of B lymphocytes responsive to specific antigenic epitope was unlikely. Compared to the homologous germline, the mutation frequency of PAL was 9% on average. Only one case might have more replacement mutations in the complementarity-determining regions than expected by chance, thus antigen-selected maturation might not take place in PAL. Intraclonal sequence heterogeneity in the V&lt;sub&gt;H&lt;\/sub&gt; gene was found in another case. From these findings, it is concluded that PAL is composed of B lymphocytes at the differentiation stage of the postgerminal center. Antigen-selected maturation might not take place in PAL, which is distinct from the majority of B cell lymphomas.<\/jats:p>","DOI":"10.1159\/000059572","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:17Z","timestamp":1050440717000},"page":"241-250","source":"Crossref","is-referenced-by-count":15,"title":["DNA Sequences of the Immunoglobulin Heavy Chain Variable Region Gene in Pyothorax-Associated Lymphoma"],"prefix":"10.1159","volume":"62","author":[{"given":"Hideaki","family":"Miwa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Takakuwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Nakatsuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Iuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Aozasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/379751a0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960703)67:1<86::AID-IJC15>3.3.CO;2-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199911113412007"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199602)178:2<122::AID-PATH486>3.0.CO;2-D"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.9337"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.1.59"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2463"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/59572","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/59572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:03:04Z","timestamp":1745467384000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000059572"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":7,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2002,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000059572","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"value":"0030-2414","type":"print"},{"value":"1423-0232","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002]]}}}