{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T23:26:43Z","timestamp":1783121203673,"version":"3.54.6"},"reference-count":3,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>Thin basement membrane disease (TBMD) and Alport syndrome, two forms of childhood nephritis, have generally been considered to be hereditary diseases. In Alport syndrome, several reports have demonstrated pathogenic mutations of the genes encoding type IV collagen \u03b13, 4 and\/or 5 chain [\u03b13, 4 and\/or 5(IV)]. Previous immunohistochemical studies indicated that these antigens were absent from the glomerular basement membrane (GBM) in Alport syndrome, whilst a normal labeling pattern was maintained in TBMD. In order to understand the role of the \u03b13, 4 and\/or 5(IV) antigens in TBMD, we used confocal laser scanning microscopy (CLSM) to examine cryosections of renal biopsies from 12 children with TBMD and 11 control children with IgA nephropathy (IgAN) without proteinuria. All tissue sections were stained with a mixture of FITC-conjugated rat monoclonal antibodies directed against human \u03b13(IV), \u03b14(IV) or \u03b15(IV) and a Texas red-conjugated rat monoclonal antibody raised against human \u03b12(IV). CLSM was performed and quantitative analysis of the ratio of the staining signal for \u03b13(IV), \u03b14(IV) or \u03b15(IV) to \u03b12(IV) [\u03b13(IV), \u03b14(IV) or \u03b15(IV)\/\u03b12(IV)] along the GBM was determined. The average number of pixels for \u03b13(IV), \u03b14(IV) or \u03b15(IV)\/\u03b12(IV) was 3.52 \u00b1 1.49, 3.54 \u00b1 1.25 and 1.09 \u00b1 0.49 in TBMD and 3.62 \u00b1 1.46, 3.99 \u00b1 1.53 and 1.77 \u00b1 0.47 in control subjects, respectively. Statistical analysis indicated that \u03b15(IV)\/\u03b12(IV) ratio was significantly lower (p &lt; 0.01) in children with TBMD compared to controls. These findings raise the possibility that TBMD might be caused by an abnormality of the \u03b15(IV) antigen along the GBM.<\/jats:p>","DOI":"10.1159\/000063288","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:17Z","timestamp":1050440717000},"page":"271-278","source":"Crossref","is-referenced-by-count":11,"title":["Quantitative Analysis of Glomerular Type IV Collagen Alpha3\u20135 Chain Expression in Children with Thin Basement Membrane Disease"],"prefix":"10.1159","volume":"92","author":[{"given":"Taku","family":"Ueda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Akazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Maruhashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironobu","family":"Shimoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitugu","family":"Sakagami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Takagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidekazu","family":"Kamitsuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Naito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Sado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Yoshioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2002,9,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1997)9:6<477::AID-HUMU1>3.3.CO;2-H"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000013499"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1086\/302106"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/63288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/63288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:06:45Z","timestamp":1745467605000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000063288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":3,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2002,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000063288","archive":["Portico"],"relation":{},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002]]}}}