{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:12:34Z","timestamp":1745554354650,"version":"3.40.4"},"reference-count":1,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>Detection of methicillin-resistant staphylococci is critical for the management of infected patients in the hospital. A total of 55 nonreplicated clinical isolates of staphylococci (31 &lt;i&gt;Staphylococcus aureus &lt;\/i&gt;and 24 coagulase-negative staphylococci; CNS) collected during a one-year period and expressing low-level resistance to methicillin (oxacillin MIC of 2\u20134 mg\/l for &lt;i&gt;S. aureus&lt;\/i&gt; and 0.5\u20134 mg\/l for CNS) were studied. &lt;i&gt;mec&lt;\/i&gt; determinants and overproduction of \u03b2-lactamase were investigated and pulsed-field gel electrophoresis (PFGE) was applied as a typing method. Twenty-four &lt;i&gt;S. aureus&lt;\/i&gt; isolates and 19 CNS carried the &lt;i&gt;mecA&lt;\/i&gt; gene. The presence of &lt;i&gt;mecR1\/mecI&lt;\/i&gt; and &lt;i&gt;blaR1\/blaI&lt;\/i&gt; genes correlated with the expression of low-level methicillin resistance in CNS. Four &lt;i&gt;mecA-&lt;\/i&gt;negative isolates (2 &lt;i&gt;S. aureus&lt;\/i&gt; and 2 CNS) overproduced \u03b2-lactamase. PFGE revealed the presence of 2 major clonal types in &lt;i&gt;mecA&lt;\/i&gt;-positive &lt;i&gt;S. aureus&lt;\/i&gt; isolates, and 3 in CNS. Low-level methicillin resistance of staphylococci is correlated with the presence of the &lt;i&gt;mecA&lt;\/i&gt; gene and overproduction of \u03b2-lactamase.<\/jats:p>","DOI":"10.1159\/000063871","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:17Z","timestamp":1050440717000},"page":"174-181","source":"Crossref","is-referenced-by-count":6,"title":["Presence of &lt;i&gt;mec&lt;\/i&gt; Genes and Overproduction of Beta-Lactamase in the Expression of Low-Level Methicillin Resistance among Staphylococci"],"prefix":"10.1159","volume":"48","author":[{"given":"E.","family":"Petinaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Arvaniti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Bartzavali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Dimitracopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Spiliopoulou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,9,13]]},"reference":[{"doi-asserted-by":"publisher","key":"ref1","DOI":"10.1089\/10766290152652855"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/63871","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/63871","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:13:44Z","timestamp":1745468024000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000063871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":1,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2002,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000063871","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"type":"print","value":"0009-3157"},{"type":"electronic","value":"1421-9794"}],"subject":[],"published":{"date-parts":[[2002]]}}}