{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:02:57Z","timestamp":1758819777544,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Some &lt;i&gt;Helicobacter&lt;\/i&gt; species colonize the intestinal tract. To explore the possible relation between &lt;i&gt;Helicobacter &lt;\/i&gt;spp. and gallbladder disorders, we have investigated their presence in bile of patients with biliary obstruction and dilatation of the bile ducts. &lt;i&gt;Methods:&lt;\/i&gt; Bile was sampled from 31 Dutch patients with biliary obstruction identified by jaundice and dilatation of the bile ducts on ultrasound. Samples (n = 31) were obtained immediately following cannulation of the common bile duct (CBD) by endoscopic retrograde cholangiopancreatography (ERCP) (n = 29) or by peri-operative puncture of the galbladder (n = 2). DNA was isolated from bile by binding to diatoms. &lt;i&gt;Helicobacter&lt;\/i&gt; spp. were detected by a sensitive (detection limit 1 CFU per reaction tube) 16S rDNA PCR with genus-specific primers. Duplicate samples were spiked with &lt;i&gt;Helicobacter pylori&lt;\/i&gt; DNA and subjected to PCR in order to check for inhibition. &lt;i&gt;Results:&lt;\/i&gt; 28 patients had CBD stones (bile collected by ERCP (n = 26) or operatively (n = 2)), 2 had a pancreatic head tumor, and in 1 no abnormalities were found. In 1 of 21 amplifiable bile samples (10\/31 inhibited) from Dutch patients with CBD stones, &lt;i&gt;H. pylori&lt;\/i&gt; 16S rDNA was found. &lt;i&gt;Conclusion:&lt;\/i&gt; Our data indicate that CBD stones in Dutch patients are not associated with the presence of &lt;i&gt;Helicobacter&lt;\/i&gt; spp. in bile.<\/jats:p>","DOI":"10.1159\/000065595","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:17Z","timestamp":1050440717000},"page":"89-91","source":"Crossref","is-referenced-by-count":10,"title":["&lt;i&gt;Helicobacter&lt;\/i&gt; Species Are Not Detectable by16S rDNA PCR in Bile from Dutch Patients with Common Bile Duct Stones"],"prefix":"10.1159","volume":"66","author":[{"given":"Robert","family":"Roosendaal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst J.","family":"Kuipers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina M.J.E.","family":"Vandenbroucke-Grauls","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes G.","family":"Kusters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,11,20]]},"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65595","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65595","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:32:04Z","timestamp":1745469124000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000065595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2002,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000065595","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2002]]}}}