{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:28:08Z","timestamp":1775003288941,"version":"3.50.1"},"reference-count":8,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2002]]},"abstract":"<i>Background:<\/i> Bee and wasp venom extracts contain potent allergens capable of inducing severe clinical reactions. To analyze immediate-type hypersensitivity to defined hymenoptera venom components, a recently developed in vitro test was applied that is based on the upregulation of CD203c expression on basophils. <i>Methods:<\/i> CD203c expression on blood basophils of 9 healthy donors and 39 patients allergic to bee and\/or wasp venom was analyzed by flow cytometry before and after activation with the purified bee venom allergens phospholipase A<sub>2<\/sub> (Api m 1), hyaluronidase (Api m 2) and melittin (Api m 4), or the purified wasp venom allergens phospholipase A<sub>1<\/sub> (Ves v 1), hyaluronidase (Ves v 2) and the recombinant antigen 5 (Ves v 5). Venom-induced CD203c upregulation on basophils was compared with skin tests and assessment of specific IgE. Basophils of nonresponders were preincubated with 10 ng\/ml interleukin-3 (IL-3) prior to allergen stimulation. <i>Results:<\/i> CD203c upregulation on basophils was induced by defined hymenoptera venom components in 35\/39 patients with a diagnosed allergy to wasp and\/or bee venom. Twenty-seven of the 34 tested patients with wasp allergy showed CD203c upregulation in response to Ves v 5, 26 of these patients also reacted with Ves v 2 and 17 with Ves v 1. Nine of 13 patients with bee allergy reacted with Api m 1, 13 individuals with Api m 2 and none of these patients with the minor allergen Api m 4. A diagnosed wasp allergy could also be confirmed in the prestimulated basophils (IL-3) of 2 nonresponder individuals who failed to upregulate CD203c in response to IgE receptor cross-linking prior to culture with IL-3. <i>Conclusions:<\/i> Flow-cytometric determination of CD203c upregulation on basophils activated by molecularly defined allergens is a powerful method to identify the precise allergen reactivity in sensitized individuals.<\/jats:p>","DOI":"10.1159\/000065875","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"160-168","source":"Crossref","is-referenced-by-count":47,"title":["Individual Hymenoptera Venom Compounds Induce Upregulation of the Basophil Activation Marker Ectonucleotide Pyrophosphatase\/Phosphodiesterase 3 (CD203c) in Sensitized Patients"],"prefix":"10.1159","volume":"129","author":[{"given":"Marc","family":"Binder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Fierlbeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te Piao","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Valent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-J\u00f6rg","family":"B\u00fchring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,10,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000049531"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90085-X"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.110928"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.125257"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.10.3303"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000024440"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2460"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(83)90069-X"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65875","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65875","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:10:28Z","timestamp":1745464228000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000065875"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":8,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2002,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000065875","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002]]}}}