{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:09:16Z","timestamp":1745554156546,"version":"3.40.4"},"reference-count":5,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmologica"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>&lt;i&gt;Purpose:&lt;\/i&gt; To investigate a rapid and sensitive polymerase chain reaction (PCR)-based assay for the detection of adenoviral infections in conjunctival swabs. &lt;i&gt;Methods:&lt;\/i&gt; Degenerate primers derived from the adenoviral hexon gene were designed for amplification of a 302-bp product by PCR. In 15 ocular swab specimens, using a simple sample preparation method, PCR was compared with a commercially available direct immunofluorescence test. &lt;i&gt;Results:&lt;\/i&gt; Fourteen samples of patients with clinically diagnosed adenoviral keratoconjunctivitis, when screened by direct immunofluorescence, were positive for adenoviral protein. In all samples positive by immunofluorescence, the 302-bp product was amplifiable. The sample negative by direct immunofluorescence was positive using PCR. &lt;i&gt;Conclusion:&lt;\/i&gt; PCR using degenerate primers proved to have several advantages over current diagnostic techniques. It offers considerable improvement in sensitivity over immunoassays and speed over tissue culture isolation and is a highly potential tool for the diagnosis of adenoviral ocular infections.<\/jats:p>","DOI":"10.1159\/000066175","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:40Z","timestamp":1050440740000},"page":"329-332","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Polymerase Chain Reaction for the Detection of Adenoviruses in Conjunctival Swab Specimens Using Degenerate Primers in Comparison with Direct Immunofluorescence"],"prefix":"10.1159","volume":"216","author":[{"given":"Ursula","family":"Weitgasser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva Maria","family":"Haller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosuf","family":"El-Shabrawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,11,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(200003)60:3<342::AID-JMV14>3.0.CO;2-0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199703)51:3<198::AID-JMV9>3.0.CO;2-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0197(98)00021-X"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(90)90020-G"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199607)49:3<187::AID-JMV5>3.0.CO;2-0"}],"container-title":["Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/66175","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/66175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:13:13Z","timestamp":1745464393000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000066175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":5,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2002,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000066175","archive":["Portico"],"relation":{},"ISSN":["0030-3755","1423-0267"],"issn-type":[{"type":"print","value":"0030-3755"},{"type":"electronic","value":"1423-0267"}],"subject":[],"published":{"date-parts":[[2002]]}}}