{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:08:50Z","timestamp":1760897330119,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2002,12,4]],"date-time":"2002-12-04T00:00:00Z","timestamp":1038960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,12,4]],"date-time":"2002-12-04T00:00:00Z","timestamp":1038960000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2003]]},"abstract":"<i>Objective:<\/i><i>TP53<\/i> mutations are the most frequent genetic alterations in colon cancer. We studied whether the recently developed oligonucleotide microarray technique, GeneChip <i>p53<\/i> assay, can be applied to sensitive detection of <i>TP53<\/i> gene mutations in surgical specimens from colon cancer patients. <i>Methods:<\/i><i>TP53<\/i> gene mutations in exons 2\u201311 in 20 colon cancers and the corresponding histopathologically normal mucosa at the surgical margins were assessed by GeneChip <i>p53<\/i> assay, and the results were further evaluated by direct sequencing of the involved exon or by mutant-allele-specific amplification (MASA). The expression of TP53 protein was also evaluated immunohistochemically and the result was compared with the gene alteration. <i>Results:<\/i> The GeneChip <i>p53<\/i> assay detected<i> TP53<\/i> mutations in 65% of primary cancers; 61% of the mutations were within the evolutionarily conserved regions, and 46% of the mutations were within the zinc-binding domains (regions of loop 2 and loop 3). Direct sequencing confirmed these mutations. Immunohistochemical examination detected TP53 protein overexpression in 47% of primary cancers, but this protein did not accumulate with all types of <i>TP53<\/i> mutations. In addition, the GeneChip assay detected a mutation identical to that in the primary tumor in 2 samples from the surgical margins, and MASA confirmed both mutations, implying the presence of occult cancer cells. <i>Conclusion:<\/i> The GeneChip <i>p53<\/i> assay is sufficiently sensitive to detect <i>TP53<\/i> mutations in surgical specimens from colon cancers and may be applicable to screening examination in clinical laboratories as a routine procedure.<\/jats:p>","DOI":"10.1159\/000066510","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"54-60","source":"Crossref","is-referenced-by-count":15,"title":["Clinical Application of Oligonucleotide Probe Array for Full-Length Gene Sequencing of <i>TP53<\/i> in Colon Cancer"],"prefix":"10.1159","volume":"64","author":[{"given":"Yasuo","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukimoto","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihito","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Ikarashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Asai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,12,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.47.1.285"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199310283291807"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(99)80071-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.13.7382"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(00)00231-4"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/342705a0"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202200"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502163320704"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/66510","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/66510","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:16:48Z","timestamp":1745464608000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000066510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,4]]},"references-count":8,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2002,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000066510","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"type":"print","value":"0030-2414"},{"type":"electronic","value":"1423-0232"}],"subject":[],"published":{"date-parts":[[2002,12,4]]}}}