{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T03:23:23Z","timestamp":1780370603123,"version":"3.54.1"},"reference-count":2,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Bacterial adherence to mucosal and epithelial cell structures is of importance for the persistence of bacteria in the airways. Cigarette smoking and chronic bronchitis are associated with increased bacterial adherence. N-Acetylcysteine (NAC) medication reduces the number of infectious exacerbations in patients with chronic bronchitis, and NAC medication has been associated with low intrabronchial bacterial numbers. &lt;i&gt;Objective:&lt;\/i&gt; We investigated whether NAC influences bacterial adherence as a possible mechanism behind its clinical effects. &lt;i&gt;Methods:&lt;\/i&gt; Highly adhering test strains of &lt;i&gt;Streptococcus pneumoniae&lt;\/i&gt; and &lt;i&gt;Haemophilus influenzae&lt;\/i&gt; were used to investigate the influence of four pharmacological compounds on adherence to oropharyngeal epithelial cells in vitro. Adhesion assays were performed both during short-term exposure to, as well as after long-time incubation with, NAC, lidocaine, hydrocortisone and terbutaline at concentrations not inhibiting bacterial growth. &lt;i&gt;Results:&lt;\/i&gt; Only NAC showed a significant inhibitory effect on adhesion of &lt;i&gt;H. influenzae&lt;\/i&gt; during short-term incubation. After long-term incubation, both NAC and hydrocortisone inhibited bacterial adhesion for both strains in a dose-dependent manner. When NAC\u2019s effect on three different strains of &lt;i&gt;S. pneumoniae&lt;\/i&gt; and four strains of &lt;i&gt;H. influenzae&lt;\/i&gt; was studied, inhibition of bacterial adhesion was found for three strains of each species. &lt;i&gt;Conclusions:&lt;\/i&gt; NAC lowers bacterial adhesion in vitro to oropharyngeal epithelial cells in doses equivalent to that is being used clinically. This effect might be a contributory mechanism behind the reduction of infectious exacerbations in chronic bronchitis patients.<\/jats:p>","DOI":"10.1159\/000067473","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:26:45Z","timestamp":1050427605000},"page":"552-558","source":"Crossref","is-referenced-by-count":61,"title":["Inhibitory Effect of N-Acetylcysteine on Adherence of &lt;i&gt;Streptococcus pneumoniae&lt;\/i&gt; and &lt;i&gt;Haemophilus influenzae&lt;\/i&gt; to Human Oropharyngeal Epithelial Cells in vitro"],"prefix":"10.1159","volume":"67","author":[{"given":"Gerdt C.","family":"Riise","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingemar","family":"Qvarfordt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Larsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoria","family":"Eliasson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengt A.","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2000,10,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07101737"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07101759"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/67473","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/67473","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:24:31Z","timestamp":1745465071000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000067473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":2,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2000,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000067473","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"value":"0025-7931","type":"print"},{"value":"1423-0356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000]]}}}