{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:27Z","timestamp":1745554227499,"version":"3.40.4"},"reference-count":9,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[2002]]},"abstract":"<i>Background\/Aim:<\/i> Patients with idiopathic focal segmental glomerulosclerosis (FSGS) often develop a recurrence of the disease after kidney transplantation. In a number of FSGS patients, plasmapheresis and immunoadsorption procedures have been shown to transiently reduce proteinuria and are thought to do this by eliminating a circulating factor. Direct cellular effects of eluates from immunoadsorption procedures on podocytes, the primary target of injury in FSGS, have not yet been reported. <i>Methods:<\/i> Eluates were derived from antibody-based immunoadsorption of a patient suffering from primary FSGS, a patient with systemic lupus erythematosus, and a healthy volunteer. The cytosolic free Ca<sup>2+<\/sup> concentration ([Ca<sup>2+<\/sup>]<sub>i<\/sub>) of differentiated podocytes was measured by single-cell fura-2 microfluorescence measurements. Free and total immunoreactive kinin levels were measured by radioimmunoassay. <i>Results:<\/i> FSGS eluates increased the [Ca<sup>2+<\/sup>]<sub>i<\/sub> levels concentration dependently (EC<sub>50<\/sub> 0.14 mg\/ml; n = 3\u201319). 1 mg\/ml eluate increased the [Ca<sup>2+<\/sup>]<sub>i<\/sub> values reversibly from 82 \u00b1 12 to 1,462 \u00b1 370 nmol\/l, and then they returned back to 100 \u00b1 16 nmol\/l (n = 19). The eluate-induced increase of [Ca<sup>2+<\/sup>]<sub>i<\/sub> consisted of an initial Ca<sup>2+<\/sup> peak followed by a Ca<sup>2+<\/sup> plateau which depended on the extracellular Ca<sup>2+<\/sup> concentration. The eluate-induced increase of [Ca<sup>2+<\/sup>]<sub>i<\/sub> was inhibited by the specific B<sub>2<\/sub> kinin receptor antagonist Hoe\u00a0140 in a concentration-dependent manner (IC<sub>50<\/sub> 2.47\u00a0nmol\/l). In addition, prior repetitive application of bradykinin desensitized the effect of eluate on [Ca<sup>2+<\/sup>]<sub>i<\/sub>. A colonic epithelial cell line not reacting to bradykinin did not respond to eluate either (n = 6). Similar to FSGS eluates, the eluate preparations of both the systemic lupus patient and the healthy volunteer led to a biphasic, concentration-dependent [Ca<sup>2+<\/sup>]<sub>i<\/sub> increase in podocytes which again was inhibited by Hoe 140. Free kinins were detected in all eluate preparations. <i>Conclusion:<\/i> The procedure of antibody-based immunoadsorption leads to kinin in the eluate which elevates the [Ca<sup>2+<\/sup>]<sub>i<\/sub> level of podocytes via B<sub>2<\/sub> kinin receptors.<\/jats:p>","DOI":"10.1159\/000068697","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:40Z","timestamp":1050440740000},"page":"384-393","source":"Crossref","is-referenced-by-count":2,"title":["Eluate Derived by Extracorporal Antibody-Based Immunoadsorption Elevates the Cytosolic Ca<sup>2+<\/sup> Concentration in Podocytes via B<sub>2<\/sub> Kinin Receptors"],"prefix":"10.1159","volume":"25","author":[{"given":"Karl-Georg","family":"Fischer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias Bruno","family":"Huber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Henger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin","family":"Fink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eckhard","family":"Schwertfeger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars Christian","family":"Rump","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Pavenst\u00e4dt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,2,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199401063300102"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199604043341402"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00369.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1081\/JDI-100101955"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.1.193"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5438.312"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050192"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.32.1.511"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00837.x"}],"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/68697","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/68697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:37:09Z","timestamp":1745465829000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000068697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":9,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2002,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000068697","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"type":"print","value":"1420-4096"},{"type":"electronic","value":"1423-0143"}],"subject":[],"published":{"date-parts":[[2002]]}}}