{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:28Z","timestamp":1745554228689,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>&lt;i&gt;Mycobacterium avium&lt;\/i&gt; subsp. &lt;i&gt;paratuberculosis (MAP)&lt;\/i&gt; is a member of the &lt;i&gt;Mycobacterium avium&lt;\/i&gt; complex (MAC) and causes the inflammatory bowel disease, Johne\u2019s disease, in livestock. &lt;i&gt;MAP&lt;\/i&gt; has also been implicated as the causative agent of a similar disease, Crohn\u2019s disease, in humans. One of three major genetic differences between &lt;i&gt;MAP&lt;\/i&gt; and non-pathogenic MAC is the 6,496-bp GS element. Based on the output from freely available protein sequence and structural bioinformatics tools, and the close homology of GS genes with the &lt;i&gt;ser2&lt;\/i&gt; region of the closely related &lt;i&gt;Mycobacterium avium&lt;\/i&gt; subsp. &lt;i&gt;avium (MAA),&lt;\/i&gt; we predict that GS encoded enzymes are involved in the biosynthesis of GDP-fucose, and the addition to, and modification of fucose on, the oligosaccharide moiety of GPL. GPL is a major constituent of the cell wall of the MAC and has immunomodulatory properties. Therefore, the enzymes involved in its synthesis may provide novel drug targets against &lt;i&gt;MAP&lt;\/i&gt; and other pathogenic MAC members.<\/jats:p>","DOI":"10.1159\/000068723","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:40Z","timestamp":1050440740000},"page":"57-66","source":"Crossref","is-referenced-by-count":7,"title":["Use of Bioinformatics to Predict a Function for the GS Element in &lt;i&gt;Mycobacterium avium&lt;\/i&gt; Subspecies &lt;i&gt;paratuberculosis&lt;\/i&gt;"],"prefix":"10.1159","volume":"5","author":[{"given":"Joe M.","family":"Sheridan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim J.","family":"Bull","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Hermon-Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,3,31]]},"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/68723","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/68723","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:37:15Z","timestamp":1745465835000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000068723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2003,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000068723","archive":["Portico"],"relation":{},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"type":"print","value":"2673-1665"},{"type":"electronic","value":"2673-1673"}],"subject":[],"published":{"date-parts":[[2003]]}}}