{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:06:51Z","timestamp":1760216811691,"version":"3.40.4"},"reference-count":9,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dev Neurosci"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>Selective inhibition of neuronal and inducible nitric oxide synthase (NOS) with 2-iminobiotin previously showed a reduction in brain cell injury. In the present study, we investigated the effects of 2-iminobiotin treatment on insulin-like growth factor-1 (IGF-1) expression, caspase activity and cytokine expression in a newborn piglet model of perinatal hypoxia-ischaemia. Newborn piglets were subjected to 1 h of hypoxia-ischaemia and were treated intravenously with vehicle or 2-iminobiotin. Vehicle-treated piglets showed reduced IGF-1 mRNA expression and increased caspase-3 activity and DNA fragmentation. 2-Iminobiotin treatment, administered immediately upon reperfusion, prevented these observations. No differences in caspase-8 and -9 activity and cytokine [interleukin (IL)-1\u03b1\/\u03b2, IL-6, tumour necrosis factor (TNF)-\u03b1, transforming growth factor (TGF)-\u03b2] mRNA expression were demonstrated between vehicle- and 2-iminobiotin-treated piglets at 24 h following hypoxia-ischaemia. IGF-1 mRNA correlated inversely with caspase-3 and transferase-mediated dUTP-biotin in situ nick end labelling score in the cortex, but positively with caspase-8. Cytokine mRNA did not correlate with IGF-1 mRNA, caspase-3 activity or DNA fragmentation. The present results indicate that the previously demonstrated neuroprotective effect of 2-iminobiotin treatment after perinatal hypoxia-ischaemia coincided with a preservation of the endogenous IGF-1 production and reduced caspase-3 activity, but not with a significant decrease in cytokine production.<\/jats:p>","DOI":"10.1159\/000069045","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:11:24Z","timestamp":1050408684000},"page":"396-404","source":"Crossref","is-referenced-by-count":17,"title":["Effects of Selective Nitric Oxide Synthase Inhibition on IGF-1, Caspases and Cytokines in a Newborn Piglet Model of Perinatal Hypoxia-Ischaemia"],"prefix":"10.1159","volume":"24","author":[{"given":"Cacha","family":"Peeters-Scholte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna","family":"Koster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyn","family":"van den Tweel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klas","family":"Blomgren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Hamers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changlian","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"van Buul-Offers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Hagberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"van Bel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cobi","family":"Heijnen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floris","family":"Groenendaal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,3,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000014599"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(92)90151-Z"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200102000-00001"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.4.571"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000047087"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1210\/en.140.12.5876"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200205000-00005"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199910000-00006"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199609)168:3<499::AID-JCP2>3.0.CO;2-K"}],"container-title":["Developmental Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:41:24Z","timestamp":1745466084000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000069045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":9,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2003,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000069045","archive":["Portico"],"relation":{},"ISSN":["0378-5866","1421-9859"],"issn-type":[{"type":"print","value":"0378-5866"},{"type":"electronic","value":"1421-9859"}],"subject":[],"published":{"date-parts":[[2002]]}}}