{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T19:27:34Z","timestamp":1769023654709,"version":"3.49.0"},"reference-count":1,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>Mutations in the gene optineurin &lt;i&gt;(OPTN)&lt;\/i&gt; have been associated with primary-open angle glaucoma. Here we present a study on the level of &lt;i&gt;OPTN&lt;\/i&gt; gene expression in the human trabecular meshwork in response to increased perfusion pressure in the anterior chamber perfusion model of the human eye. Perfusion pressure was raised from 10 to 30 mm Hg for periods ranging between 1 and 24 h. &lt;i&gt;OPTN&lt;\/i&gt; transcript levels in the trabecular meshwork were determined using real-time quantitative polymerase chain reaction. The results show no statistically significant alteration of the &lt;i&gt;OPTN&lt;\/i&gt; transcript level after raising the pressure. Moreover, no changes were detected in the transcript levels of the 3 known &lt;i&gt;OPTN&lt;\/i&gt; isoforms. This result shows that enhanced pressure levels do not lead to rapid changes in gene expression levels of &lt;i&gt;OPTN&lt;\/i&gt; in human trabecular meshwork. This suggests that alterations in &lt;i&gt;OPTN&lt;\/i&gt; gene expression are not involved in the mechanisms regulating aqueous humor outflow after an increase in intraocular eye pressure.<\/jats:p>","DOI":"10.1159\/000069133","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:11:24Z","timestamp":1050408684000},"page":"93-96","source":"Crossref","is-referenced-by-count":10,"title":["Optineurin Gene Expression Level in Human Trabecular Meshwork Does Not Change in Response to Pressure Elevation"],"prefix":"10.1159","volume":"35","author":[{"given":"Willem","family":"Kamphuis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Schneemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,3,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)04240-3"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69133","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:42:39Z","timestamp":1745466159000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000069133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":1,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2003,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000069133","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"value":"0030-3747","type":"print"},{"value":"1423-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}