{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:20:04Z","timestamp":1758709204808,"version":"3.40.4"},"reference-count":5,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>&lt;i&gt;Background: &lt;\/i&gt;Caffeine has shown an inhibitory role in invasion and proliferation in melanoma pulmonary metastasis as well as in high-grade tissue sarcoma. However, little is known about its mechanism and possible role in metastatic cell lines. &lt;i&gt;Materials and Methods:&lt;\/i&gt; B16F10 and B16F1 cell lines of high and low metastatic potential were treated with caffeine at different time intervals with different doses. Reduced glutathione, glutathione S-transferase and lipid peroxides were estimated to evaluate the effect of caffeine. &lt;i&gt;Results:&lt;\/i&gt; Caffeine treatment showed glutathione depletion and increased lipid peroxidation with higher glutathione S-transferase activity in both B16F10 and B16F1 cell lines. However the effect of caffeine was dependent on the time factor as well as on the dose. &lt;i&gt;Conclusions:&lt;\/i&gt; Caffeine was an effective inhibitor of metastatic activity. Glutathione depletion in conjunction with increased lipid peroxidation was a potent indicator in the regulation of metastatic behavior of B16F10 and B16F1 melanoma cell lines.<\/jats:p>","DOI":"10.1159\/000069785","type":"journal-article","created":{"date-parts":[[2003,4,25]],"date-time":"2003-04-25T13:11:41Z","timestamp":1051276301000},"page":"71-75","source":"Crossref","is-referenced-by-count":5,"title":["Potentiation of Lipid Peroxidation in B16F10 and B16F1 Melanoma Cells by Caffeine, a Methylxanthine Derivative: Relationship to Intracellular Glutathione"],"prefix":"10.1159","volume":"49","author":[{"given":"Vivek","family":"Shukla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajiv P.","family":"Gude","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,5,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000028403"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20010520)95:3<152::AID-IJC1026>3.0.CO;2-S"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000025380"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(80)90023-8"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000007322"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69785","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69785","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:13:59Z","timestamp":1745464439000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000069785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":5,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2003,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000069785","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"type":"print","value":"0009-3157"},{"type":"electronic","value":"1421-9794"}],"subject":[],"published":{"date-parts":[[2003]]}}}