{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:29:38Z","timestamp":1783132178967,"version":"3.54.6"},"reference-count":17,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Drug-induced toxic epidermal necrolysis (TEN) is a rare life-threatening disease characterized by the extensive destruction of the epidermis contrasting with the discreteness of lymphoid cell infiltration. The precise pathomechanism of the disease remains unclear. &lt;i&gt;Methods:&lt;\/i&gt; Skin specimens and peripheral and thoracic lymph nodes (LNs) were collected from 2 TEN patients. They were examined by conventional histology and immunohistochemistry using antibodies directed to factor XIIIa (dermal dendrocytes), CD1a (Langerhans cells), CD15 (granulocytes), CD20 (B lymphocytes), CD45RO (activated T lymphocytes), CD68 (macrophages) and the proliferation marker Ki-67. LNs from patients with mycosis fungoides, from subjects dead from acute cardiac failure or traumatic injuries, as well as metastasis-free sentinel LNs of cutaneous melanoma served as positive and negative controls. &lt;i&gt;Results:&lt;\/i&gt; TEN LNs showed absence of germinal centers but a moderate hyperplasia of the paracortical T cell zone. Immunohistochemistry did not reveal any distinctive aspect in LN cellular densities between TEN and other control conditions except for the factor-XIIIa+ dendritic cells which were dramatically reduced in numbers in TEN LNs. This rarefaction in LNs contrasted with the great number of these cells in the skin of the same patients. &lt;i&gt;Conclusion:&lt;\/i&gt; The structure of TEN LNs rules out the involvement of an antibody-mediated response. As dendritic factor-XIIIa+ cells are involved in most cutaneous T-cell-mediated responses, their depletion in TEN LNs could explain the sparse lymphoid cell infiltrate in lesional TEN skin.<\/jats:p>","DOI":"10.1159\/000069961","type":"journal-article","created":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T07:40:46Z","timestamp":1054107646000},"page":"374-378","source":"Crossref","is-referenced-by-count":11,"title":["Factor-XIIIa-Positive Dendrocytes in Drug-Induced Toxic Epidermal Necrolysis (Lyell\u2019s Syndrome): Paradoxical Activation in Skin and Rarefaction in Lymph Nodes"],"prefix":"10.1159","volume":"206","author":[{"given":"Philippe","family":"Paquet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Quatresooz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rald E.","family":"Pi\u00e9rard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2003,6,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000048174"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199704000-00005"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000018112"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-200010000-00005"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.129.4.466"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000051751"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199411103311906"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.130.5.605"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00189.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.4.627"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000018108"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2000.17717.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199912093412407"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199809)28:09<2760::AID-IMMU2760>3.0.CO;2-N"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.1.51"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.2.373"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.3.447"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69961","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/69961","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:16:21Z","timestamp":1745464581000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000069961"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":17,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2003,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000069961","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}