{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:30:52Z","timestamp":1791268252637,"version":"4.1.0"},"reference-count":5,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>Pineal gland G-protein coupled P2Y&lt;sub&gt;1&lt;\/sub&gt; receptors potentiate noradrenaline-induced N\u2032-acetylserotonin production, a long term response which occurs after 5 h incubation. In the current study we show that a short-term effect of stimulation of P2Y&lt;sub&gt;1&lt;\/sub&gt; receptors is the increase in extracellular acidification rate (ECAR), which is mediated by an increase in intracellular calcium concentration ([Ca&lt;sup&gt;2+&lt;\/sup&gt;]&lt;sub&gt;i&lt;\/sub&gt;). The pD&lt;sub&gt;2&lt;\/sub&gt; values for ATP (3.06 \u00b1 0.12)-induced ECAR increase was significantly smaller (p &lt; 0.01) than that for ADP (3.64 \u00b1 0.18), 2MeSATP (3.56 \u00b1 0.02) and 2MeSADP (3.65 \u00b1 0.13). The selective P2Y&lt;sub&gt;1&lt;\/sub&gt; receptor antagonists A3\u2032P-5\u2032P and A3\u2032P-5\u2032PS inhibited the increase in ECAR-induced by ADP. Clamping [Ca&lt;sup&gt;2+&lt;\/sup&gt;]&lt;sub&gt;i&lt;\/sub&gt; with BAPTA (30 and 50 \u00b5mol\/l) led to inhibition of ADP-induced increase in ECAR. Agonist and antagonist data indicate P2Y&lt;sub&gt;1&lt;\/sub&gt; activation leads to a [Ca&lt;sup&gt;2+&lt;\/sup&gt;]&lt;sub&gt;i&lt;\/sub&gt;-dependent acidification of the extracellular medium.<\/jats:p>","DOI":"10.1159\/000071264","type":"journal-article","created":{"date-parts":[[2003,7,29]],"date-time":"2003-07-29T09:18:09Z","timestamp":1059470289000},"page":"33-37","source":"Crossref","is-referenced-by-count":12,"title":["P2Y&lt;sub&gt;1&lt;\/sub&gt; Receptor Activation Enhances the Rate of Rat Pinealocyte-Induced Extracellular Acidification via a Calcium-Dependent Mechanism"],"prefix":"10.1159","volume":"69","author":[{"given":"Zulma Silva","family":"Ferreira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia Regina S.","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Spray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina P.","family":"Markus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2003,8,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00416-7"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1006\/smns.1996.0026"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)00823-8"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/35051599"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900496"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71264","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:29:45Z","timestamp":1745465385000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":5,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2003,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071264","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}