{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T16:58:13Z","timestamp":1758473893823,"version":"3.40.4"},"reference-count":9,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>Background\/Aims: Microcirculatory disturbances following small intestinal ischaemia-reperfusion (I\/R) injury lead to tissue damage that may affect short- and long-term outcome after transplantation. The immunosuppressive drug Tacrolimus (FK506) attenuates I\/R injury in a number of organs, raising the possibility that it might be able to control both I\/R injury and rejection after small bowel transplantation. However, its effects on intestinal I\/R injury have not been evaluated. Methods: PVG rats were subjected to 30 min intestinal ischaemia. Animals received Tacrolimus (1 mg\/kg i.p.) 4 and 1 h prior to ischaemia. The mucosa was visualised in an exteriorised ileal segment using in vivo microscopy. FITC-BSA or Acridine orange was used to quantitate macromolecular leak (MML) and leucocyte adhesion respectively every 15\u00a0min for 2 h during reperfusion. Heart rate and mean blood pressure (mBP) were monitored throughout the experiment. Results: Ten of 12 untreated animals subjected to intestinal I\/R injury failed to survive the 2-hour reperfusion period. MML and leucocyte adhesion were increased in untreated animals (p &lt; 0.001) and blood flow stasis eventually ensued. Similar results were obtained for Tacrolimus pre-treated I\/R animals, with 10 of 12 animals again failing to survive reperfusion. Conclusions: Despite previous evidence that Tacrolimus reduces I\/R injury in other organs, it did not improve survival rates or prevent villus microcirculatory disturbances following intestinal I\/R injury. The severity of microcirculatory damage suffered by the small intestine highlights the importance of alternative therapies to combat I\/R in this organ.<\/jats:p>","DOI":"10.1159\/000071295","type":"journal-article","created":{"date-parts":[[2003,7,11]],"date-time":"2003-07-11T08:22:02Z","timestamp":1057911722000},"page":"154-160","source":"Crossref","is-referenced-by-count":5,"title":["Mucosal Villus Microcirculatory Disturbances Associated with Rat Intestinal Ischaemia-Reperfusion Injury Are Not Prevented by Tacrolimus"],"prefix":"10.1159","volume":"67","author":[{"given":"Neena","family":"Kalia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard F.M.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Graham","family":"Pockley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola J.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,7,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(96)90485-6"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200001000-00014"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200112270-00003"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(98)01401-8"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0829"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199810150-00028"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2099.91493"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/path.1136"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200207000-00011"}],"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/dig\/article-pdf\/67\/3\/154\/4032662\/000071295.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/dig\/article-pdf\/67\/3\/154\/4032662\/000071295.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:29:57Z","timestamp":1745465397000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":9,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2003,7,1]]},"published-print":{"date-parts":[[2003,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071295","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2003]]}}}