{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:08Z","timestamp":1745554208890,"version":"3.40.4"},"reference-count":10,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>The aim of this study was to analyze which types of T cells are at work and the specific nature of their response, using a mouse 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis model. The response of T cells to TNBS was analyzed by anti-TNBS mixed-lymphocyte reaction. T cell clones were established by limiting dilution. Phenotypes and T cell receptor (TCR) V\u03b2 of T cells were analyzed by flow cytometry. Colitis was induced by administration of TNBS enemas, and lamina propria lymphocytes were isolated and analyzed. The proliferative responses to TNBS of spleen T cells were partially inhibited by the addition of antimouse CD4 or CD8 antibodies to the mixed-lymphocyte culture. Conversely, these were inhibited by the addition of both antibodies. Flow cytometric analysis showed that TCR V\u03b214 T cells specifically increased in the CD8+ T cell population. We established CD8+ TCR V\u03b214 T cell clones which were TNBS reactive and self-restricted. Investigation using lamina propria lymphocytes in TNBS-induced colitis revealed that the rate of CD8+ TCR V\u03b214 T cells changed with histological inflammatory activity which also attained a peak on day 5 following enema administration. Both CD4+ and CD8+ T cell subsets responded to TNBS, and the rate of CD8+ TCR V\u03b214 T cells changed with histological inflammatory activity in TNBS-induced colitis.<\/jats:p>","DOI":"10.1159\/000071297","type":"journal-article","created":{"date-parts":[[2003,7,11]],"date-time":"2003-07-11T08:22:02Z","timestamp":1057911722000},"page":"170-178","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of the T Cell Receptor V\u03b2 Repertoire in 2,4,6-Trinitrobenzenesulfonic Acid Induced Colitis in Mice"],"prefix":"10.1159","volume":"67","author":[{"given":"Kazuto","family":"Kanetake","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Iwata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisato","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Hirota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyoo","family":"Nitta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatomo","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuyoshi","family":"Onitsuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Hirose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,7,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1996.0022"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90230-I"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1281"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.6.1348"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.2.600"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.7.1.145"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/345344a0"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.5.1863"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90079-7"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/341541a0"}],"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/dig\/article-pdf\/67\/3\/170\/4032680\/000071297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/dig\/article-pdf\/67\/3\/170\/4032680\/000071297.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:29:57Z","timestamp":1745465397000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":10,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2003,7,1]]},"published-print":{"date-parts":[[2003,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071297","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2003]]}}}