{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:30:15Z","timestamp":1761237015784,"version":"3.40.4"},"reference-count":27,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>Acute treatment with the diuretic furosemide (Lasix) produces a reduction in plasma Na&lt;sup&gt;+&lt;\/sup&gt; and volume as well as increased thirst and salt appetite. The resulting hypovolemia stimulates the well-known counter-regulatory physiological response from the renin-angiotensin-aldosterone system. However, the neurochemical players underpinning the behavioral responses of thirst and salt appetite are less clear. Previously, we have reported that salt-replete deoxycorticosterone (DOCA) treatment activates mesolimbic structures associated with reward and goal-seeking behavior. The present study was designed to test whether the same brain regions are affected in a salt-depleted state. In experiment\u00a01, two groups of adult male Sprague-Dawley (SD) rats were injected with Lasix (10\u00a0mg\/rat, s.c.) and 18\u00a0h later were allowed access either to 2% NaCl solution (\u2018Lasix+salt\u2019) or only to tap water (\u2018Lasixnosalt\u2019) for 2\u00a0h. For comparison purposes, a third group received an isotonic saline injection instead of Lasix and was allowed access to the 2% salt solution (Vehicle). All groups were permitted 24\u00a0h access to tap water. We found no differences in dynorphin-mRNA levels in any striatal and accumbal regions among any of the treatment groups. However, as found previously in DOCA-treated rats, there were increased enkephalin (ENK)-mRNA and decreased dopamine transporter (DAT) binding levels throughout the striatum in Lasix+salt and decreased ENK-mRNA in Lasixnosalt rats versus Vehicle. In experiment\u00a02, the involvement of the ENKergic and\/or dopaminergic system was tested in rats divided into the same three groups described in experiment\u00a01. However, before access to salt or water, the Lasix+salt and the vehicle groups were administered either a \u03b4-opioid, naltrindole or a dopamine D&lt;sub&gt;2&lt;\/sub&gt; antagonist, raclopride. Only the naltrindole-treated rats showed a blunted intake of salt solution. Thus, these findings along with our neurochemical results suggest that mesolimbic enkephalin might impact salt intake through dopaminergic systems.<\/jats:p>","DOI":"10.1159\/000071312","type":"journal-article","created":{"date-parts":[[2003,7,11]],"date-time":"2003-07-11T08:22:02Z","timestamp":1057911722000},"page":"406-415","source":"Crossref","is-referenced-by-count":34,"title":["Involvement of Mesolimbic Structures in Short-Term Sodium Depletion: In situ Hybridization and Ligand-Binding Analyses"],"prefix":"10.1159","volume":"77","author":[{"given":"Louis R.","family":"Lucas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia A.","family":"Grillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"McEwen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,7,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(94)00205-F"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1079"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(99)00131-8"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.97.5.746"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.97.5.738"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/syn.1088"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.111.1.129"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.111.1.169"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.111.1.180"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.108.4.714"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000054356"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90526-8"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(93)90008-N"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(91)90249-N"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159\/000054559"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00530-7"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.2.819"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19961021)374:3<387::AID-CNE6>3.0.CO;2-Y"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)00129-4"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159\/000054706"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00137-X"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(91)90114-H"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.107.6.1005"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.111.3.606"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1037\/\/0735-7044.111.1.155"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90345-4"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91645-4"}],"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:30:03Z","timestamp":1745465403000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":27,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2003,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071312","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"type":"print","value":"0028-3835"},{"type":"electronic","value":"1423-0194"}],"subject":[],"published":{"date-parts":[[2003]]}}}