{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:11Z","timestamp":1745554211479,"version":"3.40.4"},"reference-count":16,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dev Neurosci"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>N-Tosyl-&lt;i&gt;L&lt;\/i&gt;-phenylalanyl-chloromethyl ketone (TPCK) reduces apoptosis in vitro. Pretreatment with TPCK reduces brain injury. Would treatment after injury reduce damage? Seven-day-old rats had the right carotid artery ligated and were subjected to 2.5 h of 8% oxygen and were treated intraperitoneally 3 h after hypoxia with 10\u00a0mg\/kg of TPCK or vehicle. Brain damage was measured 22 days after injury. bcl-2, bax, and cytochrome c where measured by Western blot 24 h after injury. Caspase-9 and caspase-3 activity were measured enzymatically 24\u00a0h after injury. Treatment with TPCK reduced the loss of the right hemisphere caused by injury from 27.6 \u00b1 2.8% SEM (vehicle, n = 56) to 19.8 \u00b1 2.8% (TPCK, n = 61, p &lt; 0.05). Hypoxic ischemia increased cytosolic cytochrome c from 0.25 \u00b1 0.04 to 0.4 \u00b1 0.04 optical density (OD; p &lt; 0.05), but TPCK had no effect (0.31 \u00b1 0.03 OD). TPCK reduced caspase-9 activity from 72 \u00b1 30 to 43 \u00b1 5 fluorescence units\/h\/mg (p &lt; 0.05 vs. vehicle), and caspase-3 activity from 66 \u00b1 10 to 39 \u00b1 3.7 fluorescence units\/h\/mg (p &lt; 0.05 vs. vehicle). Treatment with TPCK 3 h after hypoxic ischemia reduced brain infarct size. TPCK may act by reducing caspase-9 activation by cytochrome c.<\/jats:p>","DOI":"10.1159\/000071466","type":"journal-article","created":{"date-parts":[[2003,7,29]],"date-time":"2003-07-29T09:18:09Z","timestamp":1059470289000},"page":"34-40","source":"Crossref","is-referenced-by-count":12,"title":["Treatment of Hypoxic-Ischemic Brain Injury in Newborn Rats with TPCK 3 h after Hypoxia Decreases Caspase-9 Activation and Improves Neuropathologic Outcome"],"prefix":"10.1159","volume":"25","author":[{"given":"Yangzheng","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael H.","family":"LeBlanc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,7,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2523"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8531"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.1.25"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200105000-00010"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3470669"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.2.281"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159\/000046141"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1245"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199701000-00002"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199808000-00001"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.5.2591"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.740443.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.5.1268"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205280"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.30.21155"}],"container-title":["Developmental Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71466","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71466","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:30:44Z","timestamp":1745465444000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071466"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":16,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2003,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071466","archive":["Portico"],"relation":{},"ISSN":["0378-5866","1421-9859"],"issn-type":[{"type":"print","value":"0378-5866"},{"type":"electronic","value":"1421-9859"}],"subject":[],"published":{"date-parts":[[2003]]}}}