{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:12:43Z","timestamp":1791684763182,"version":"4.3.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2003]]},"abstract":"<i>Background:<\/i><i>Blomia tropicalis<\/i> and <i>Dermatophagoides pteronyssinus<\/i> are important mite species in Tenerife, Canary Islands, Spain. Several studies have demonstrated a variable degree of allergenic cross-reactivity in vitro. However, only a few have addressed their allergenic cross-reactivity using challenge tests. <i>Objective:<\/i> The objective of this study was to conduct conjunctival and bronchial challenge tests with <i>B. tropicalis<\/i> and <i>D. pteronyssinus<\/i> extracts in a group of 42 patients with allergic asthma and\/or rhinoconjunctivitis sensitised to house dust mites (31 females, 11 males; mean age 21.7 \u00b1 7.02 years). <i>Methods:<\/i> Prick tests using standardised extracts and specific IgE determinations using the CAP system were performed. Bronchial and\/or conjunctival challenges were conducted using freeze-dried extracts of both mite species. A patient was considered sensitive to a mite species if she\/he had a positive prick and\/or CAP test result. A total of 32 conjunctival and 15 bronchial challenges were performed with both mite species. <i>Results:<\/i> Prick tests were positive to <i>B. tropicalis<\/i> in 23 patients (54.7%) and to <i>D. pteronyssinus<\/i> in 41 (97.6%). One patient (2.4%) was exclusively sensitive to <i>B. tropicalis<\/i>. The CAP test was positive for <i>B. tropicalis<\/i> in 28 patients and for <i>D. pteronyssinus<\/i> in 41. Conjunctival challenges to <i>B. tropicalis<\/i> were positive in 20 patients (18 sensitised and 2 non-sensitised) and negative in 12 (5 sensitised and 7 non-sensitised patients). Conjunctival challenges with <i>D. pteronyssinus<\/i> were positive in all 31 <i>D. pteronyssinus<\/i>-sensitised patients who underwent conjunctival challenges. Bronchial challenges with <i>B. tropicalis<\/i> were positive in 9 sensitised patients and negative in 6 patients (2 sensitised and 4 non-sensitised). Bronchial challenges with <i>D. pteronyssinus<\/i> were positive in all patients except 1, who only reacted to <i>B. tropicalis<\/i>. <i>Conclusions:<\/i> Allergens of the mite species <i>B. tropicalis<\/i> induce positive conjunctival and bronchial challenges in <i>B. tropicalis<\/i>-sensitised individuals. Our results suggest that although there is a low to moderate degree of in vivo cross-reactivity between <i>B. tropicalis<\/i> and <i>D. pteronyssinus<\/i>, <i>B. tropicalis<\/i> seems to be a relevant source of allergens in areas where patients are exposed.<\/jats:p>","DOI":"10.1159\/000071484","type":"journal-article","created":{"date-parts":[[2003,7,29]],"date-time":"2003-07-29T09:18:09Z","timestamp":1059470289000},"page":"182-188","source":"Crossref","is-referenced-by-count":19,"title":["Skin Tests and Conjunctival and Bronchial Challenges with Extracts of <i>Blomia tropicalis<\/i> and <i>Dermatophagoides pteronyssinus <\/i>in Patients with Allergic Asthma and\/or Rhinoconjunctivitis"],"prefix":"10.1159","volume":"131","author":[{"given":"J.C.","family":"Garc\u00eda Robaina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"S\u00e1nchez Mach\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Fern\u00e1ndez-Caldas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Iraola Calvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"V\u00e1zquez Moncholi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bonnet Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"de la Torre Mor\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2003,7,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000023988"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000024193"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000049525"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000068375"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01480.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.23354.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.167"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.23746.x"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71484","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/71484","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:30:55Z","timestamp":1745465455000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000071484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":8,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2003,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000071484","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}