{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T05:00:04Z","timestamp":1755925204540,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Major allergens of oilseed rape (OSR) pollen with molecular weights of 6\/8, 14 and between 27 and 69 kD have been described. The aim of the present study was to further characterize the 14-kD allergen. &lt;i&gt;Methods:&lt;\/i&gt; The 14-kD protein was purified from OSR pollen extracts by poly-(&lt;i&gt;L&lt;\/i&gt;-proline) (PLP)-Sepharose affinity chromatography and characterized immunologically by means of allergic patients\u2019 IgE antibodies, profilin-specific rabbit antisera, Western blot and ELISA inhibition using recombinant birch profilin (rBet v 2), and skin prick testing. &lt;i&gt;Results:&lt;\/i&gt; By PLP affinity chromatography, OSR pollen profilin was purified as a single protein of 14.5 kD and further identified as a profilin by three polyclonal rabbit antisera raised against ragweed and tobacco pollen profilin and the C-terminus of birch profilin. IgE binding of a human serum pool (n = 15) and four profilin-reactive sera to nitrocellulose-blotted OSR profilin was completely inhibited by 1 \u00b5g\/ml rBet v 2 (birch profilin). Reciprocal ELISA inhibition using increasing concentrations of rBet v 2 and purified OSR profilin, respectively, showed that rBet v 2 strongly inhibits antibody binding to OSR profilin, whereas almost 100 times the amount of OSR profilin was needed to inhibit IgE binding to rBet v 2. Skin prick tests were positive (wheal \u22673 mm) with 5 \u00b5g\/ml rBet v 2 in all three patients tested, and with OSR profilin in two patients at a concentration of 50 \u00b5g\/ml. &lt;i&gt;Conclusions:&lt;\/i&gt; OSR pollen profilin shares IgE and IgG epitopes with Bet v 2 and other plant profilins and may represent a potentially relevant allergen for profilin-sensitized patients.<\/jats:p>","DOI":"10.1159\/000073712","type":"journal-article","created":{"date-parts":[[2003,11,17]],"date-time":"2003-11-17T13:22:48Z","timestamp":1069075368000},"page":"116-123","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Oilseed Rape &lt;i&gt;(Brassica napus)&lt;\/i&gt; Pollen Profilin as a Cross-Reactive Allergen"],"prefix":"10.1159","volume":"132","author":[{"given":"Margarete","family":"Focke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Hemmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Valenta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Jarisch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,11,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1309"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000023996"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.120013"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000053806"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.4.1231"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1993.03050629.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(97)00164-0"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(87)90170-4"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/73712","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/73712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:45:52Z","timestamp":1745466352000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000073712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":8,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2003,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000073712","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2003]]}}}