{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T22:48:46Z","timestamp":1767998926593,"version":"3.49.0"},"reference-count":4,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Methods to simplify the stem cell transplantation procedures are needed mainly in developing countries. We have previously shown that unprocessed leukapheresis material is useful to restore hematopoiesis after high-dose chemotherapy. &lt;i&gt;Methods:&lt;\/i&gt; Over a 10-year period in a private practice setting, we prospectively performed autotransplants using noncryopreserved and unmanipulated peripheral blood stem cells mobilized from the bone marrow to the peripheral blood by means of filgrastim and using a single-day conditioning regimen with high dose (200 mg\/m&lt;sup&gt;2&lt;\/sup&gt;) melphalan. &lt;i&gt;Results:&lt;\/i&gt; Forty-six individuals were given 50 autografts. The median age of the patients was 33 years (range 8\u201369). Twenty-two patients with acute leukemia (13 with myeloblastic and 9 with lymphoblastic leukemia), 4 with chronic myelogenous leukemia, 6 with multiple myeloma, 7 with Hodgkin\u2019s disease, 3 with non-Hodgkin\u2019s lymphoma and 4 with metastatic breast carcinoma were included. The median time to achieve &gt;0.5 \u00d7 10&lt;sup&gt;9&lt;\/sup&gt;\/l granulocytes was 14 days (range 0\u201386), whereas the median time to achieve &gt;20 \u00d7 10&lt;sup&gt;9&lt;\/sup&gt;\/l platelets was 25 days (range 0\u2013102). The 3,300-day posttransplant survival was 63%, the median posttransplant overall survival was over 3,300 days, the 3,300-day disease-free survival was 50% and the transplant-related mortality was 2%. The procedure was performed entirely on an outpatient basis in the case of 48 autografts (96%). The approximate cost of each graft was 7,500 USD. &lt;i&gt;Conclusion:&lt;\/i&gt; This simplified method to autograft patients, avoiding in-hospital stays, purging procedures and cryopreservation of the cells, is feasible and results in a substantial decrease in the cost of the autologous hematopoietic stem cell transplantation methods.<\/jats:p>","DOI":"10.1159\/000074221","type":"journal-article","created":{"date-parts":[[2003,12,19]],"date-time":"2003-12-19T09:25:47Z","timestamp":1071825947000},"page":"179-183","source":"Crossref","is-referenced-by-count":37,"title":["Results of an Autologous Noncryopreserved, Unmanipulated Peripheral Blood Hematopoietic Stem Cell Transplant Program: A Single-Institution, 10-Year Experience"],"prefix":"10.1159","volume":"110","author":[{"given":"Guillermo J.","family":"Ruiz-Arg\u00fcelles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"G\u00f3mez-Rangel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo J.","family":"Ruiz-Delgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Ruiz-Arg\u00fcelles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"P\u00e9rez-Romano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana","family":"Rivadeneyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,12,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)90795-5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199807)58:3<161::AID-AJH1>3.0.CO;2-P"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-0128(99)00042-1"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199403243301206"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74221","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:50:46Z","timestamp":1745466646000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000074221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":4,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2003,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000074221","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"value":"0001-5792","type":"print"},{"value":"1421-9662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}