{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:11:14Z","timestamp":1745554274809,"version":"3.40.4"},"reference-count":4,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2004]]},"abstract":"<i>Objective:<\/i> Functional defects in DNA repair have been shown to be associated with genomic instability followed by cancer. Recently, <i>p53R2<\/i> [p53-inducible ribonucleotide reductase (RR) small subunit 2 homologous] was identified as a novel RR gene which is directly regulated by p53 protein in the G1 and G2 phases of the cell cycle supplying nucleotides to repair damaged DNA. <i>Methods:<\/i> We performed genetic analyses of <i>p53R2 <\/i>to determine whether <i>p53R2 <\/i>alterations play significant roles in urothelial tumorigenesis. Genomic DNA from 108 primary transitional cell carcinomas (TCCs; 81 of the urinary bladder and 27 of the renal pelvis or ureter) was analyzed for mutation in the <i>p53R2 <\/i>gene by direct sequencing. We focused on three domains of the <i>p53R2 <\/i>gene: one RR small subunit signature involving codons 120\u2013146 (region 1) and two putative nuclear localization signal sequences, involving codons 149\u2013155 (region 2) and codons 163\u2013169 (region 3). In addition, a p53-binding site of 20 nucleotides in intron 1 of <i>p53R2 <\/i>was also analyzed. <i>Results:<\/i> One renal pelvic TCC (0.9%: 1\/108) had a single-base substitution in <i>p53R2<\/i> with a G to T transversion resulting in the amino acid substitution Glu136 \u2192 Asp. This base substitution was localized within the domain of exon 4 encoding the RR small subunit signature, and causes an amino acid substitution in one of the most highly conserved regions of p53R2, in which human R2 and yeast RNR2 and RNR4 proteins are highly homologous. <i>Conclusion:<\/i> This finding provides the in vivo evidence for the infrequent involvement of alterations in <i>p53R2<\/i> in<i><\/i>human urothelial TCCs.<\/jats:p>","DOI":"10.1159\/000074424","type":"journal-article","created":{"date-parts":[[2004,1,21]],"date-time":"2004-01-21T08:52:58Z","timestamp":1074675178000},"page":"103-106","source":"Crossref","is-referenced-by-count":3,"title":["Infrequent Alteration in the <i>p53R2 <\/i>Gene in Human Transitional Cell Carcinoma of the Urinary Tract"],"prefix":"10.1159","volume":"71","author":[{"given":"Hideki","family":"Hayashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutsuo","family":"Furihata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morimasa","family":"Kuwahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Kagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Shuin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Ohtsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,1,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.65.1.101"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/35003506"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10253"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/path.1082"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74424","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:52:53Z","timestamp":1745466773000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000074424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":4,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2004,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000074424","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"type":"print","value":"1015-2008"},{"type":"electronic","value":"1423-0291"}],"subject":[],"published":{"date-parts":[[2004]]}}}