{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:27:06Z","timestamp":1762874826697,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T00:00:00Z","timestamp":1072051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T00:00:00Z","timestamp":1072051200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmologica"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:p>&lt;i&gt;Purpose:&lt;\/i&gt; To detect the cytomegalovirus (CMV) genome by PCR in the aqueous humor, blood leukocytes and vitreous of patients affected by retinitis and immune recovery uveitis (IRU). &lt;i&gt;Methods:&lt;\/i&gt; A PCR for CMV genome detection was carried out with the aqueous humor, vitreous and blood leukocytes of 54 patients with retinitis, including 25 HIV-infected patients presenting CMV retinitis in different stages (active lesion 6 cases, healed lesion 14 cases and IRU 5 cases), and 29 non-HIV-infected patients (retinitis unrelated to CMV) as negative controls. &lt;i&gt;Results: &lt;\/i&gt; The CMV genome was detected in the vitreous, aqueous humor and blood leukocytes of 3 out of 6 HIV-infected patients, presenting active lesions in the retina. No CMV genome was detected in the vitreous, aqueous humor and blood leukocytes of the 5 HIV-infected patients presenting IRU. &lt;i&gt;Conclusions: &lt;\/i&gt; CMV genome detection by PCR in aqueous humor could be used as a specific and highly predictive technique for confirmation of this infection in the retina. The absence of CMV, based on the results of PCR done in clinical samples of the 5 IRU cases, does not confirm the hypothesis of a viral replication in the vitreous body and aqueous humor of these patients.<\/jats:p>","DOI":"10.1159\/000074566","type":"journal-article","created":{"date-parts":[[2003,12,19]],"date-time":"2003-12-19T09:25:47Z","timestamp":1071825947000},"page":"43-48","source":"Crossref","is-referenced-by-count":9,"title":["PCR with the Aqueous Humor, Blood Leukocytes and Vitreous of Patients Affected by Cytomegalovirus Retinitis and Immune Recovery Uveitis"],"prefix":"10.1159","volume":"218","author":[{"given":"Rubens Camargo","family":"Siqueira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Or\u00e9fice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wesley Ribeiro","family":"Campos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Tadeu M.","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2003,12,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199908200-00009"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(00)00528-6"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199707000-00008"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.2.103"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(01)01322-8"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/00006982-200007000-00012"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097\/00006982-200102000-00001"}],"container-title":["Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74566","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/74566","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:54:10Z","timestamp":1745466850000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000074566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12,22]]},"references-count":7,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2004,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000074566","archive":["Portico"],"relation":{},"ISSN":["0030-3755","1423-0267"],"issn-type":[{"type":"print","value":"0030-3755"},{"type":"electronic","value":"1423-0267"}],"subject":[],"published":{"date-parts":[[2003,12,22]]}}}