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Karger AG","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurodegener Dis"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:p>Glial-cell-line-derived neurotrophic factor (GDNF) acts as a potent survival factor for many neuronal populations, including retinal ganglion cells (RGC), indicating a potential therapeutic role of GDNF for neurological disorders. To enhance the tissue distribution and applicability of the neurotrophin, we linked it to a protein transduction domain derived from the HIV TAT protein and tested it in a well-established model for traumatic injury in the CNS: After optic nerve axotomy, the number of surviving RGCs was significantly increased in mice injected with TAT-GDNF on days 0, 3, 7, and 10 after surgery compared with GDNF- or PBS-injected animals. Moreover, TAT-GDNF reduced the number of activated caspase-3-positive cells. These results show that the neuroprotective effect of substances like neurotrophins may be enhanced by linking them to a domain that has been shown to mediate efficient transduction across biological membranes.<\/jats:p>","DOI":"10.1159\/000076669","type":"journal-article","created":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T06:15:27Z","timestamp":1081836927000},"page":"44-49","source":"Crossref","is-referenced-by-count":34,"title":["The TAT Protein Transduction Domain Enhances the Neuroprotective Effect of Glial-Cell-Line-Derived Neurotrophic Factor after Optic Nerve Transection"],"prefix":"10.1159","volume":"1","author":[{"given":"\u00dclkan","family":"Kilic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ertugrul","family":"Kilic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunnar P.H.","family":"Dietz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"B\u00e4hr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2888%2990263-2"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2F4042"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2F373339a0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-199709290-00018"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-8993%2897%2901041-X"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-4547%2819980901%2953%3A5%3C593%3A%3AAID-JNR9%3E3.0.CO%3B2-4"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-199711100-00005"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0042-6989%2897%2900364-7"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0304-3940%2897%2900517-X"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.290.5492.767"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1006%2Fnbdi.2001.0399"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1006%2Fnbdi.2001.0448"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0304-3940%2894%2990284-4"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0166-2236%2800%2901637-4"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1006%2Fmcne.2002.1165"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1006%2Fmeth.2001.1186"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-200110290-00041"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.cdd.4400538"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2F0022-510X%2893%2990086-E"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fana.10356"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1161%2F01.STR.0000066869.45310.50"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2FS1385-299X%2801%2900076-9"}],"container-title":["Neurodegenerative Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76669","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76669","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:13:26Z","timestamp":1745468006000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000076669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2004,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000076669","archive":["Portico"],"relation":{},"ISSN":["1660-2854","1660-2862"],"issn-type":[{"value":"1660-2854","type":"print"},{"value":"1660-2862","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}