{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:06:02Z","timestamp":1782399962771,"version":"3.54.5"},"reference-count":5,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2004]]},"abstract":"<i>Background:<\/i><i>Epicoccum purpurascens<\/i> (formerly <i>nigrum<\/i>) (EP), is a ubiquitous saprophytic mould found both indoors and outdoors. Several studies have reported sensitization to EP in 5\u20137 % of different populations worldwide. The diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. The crude allergen extract from EP was standardized earlier, however none of its allergens have been purified. <i>Methods:<\/i> A major allergen from spore-mycelia extract of EP was purified using concanavalin A (Con A) Sepharose chromatography, gel filtration and electro-elution. The allergen isolated was characterized for its IgE-binding ability and cross-reactivity with five well-known allergenic fungi by ELISA and immunoblot. <i>Results:<\/i> A 33.5-kD glycoprotein allergen of EP, Epi p 1, was purified to homogeneity. All the EP allergic patients\u2019 sera tested recognized this protein. Periodate modification of Epi p 1 showed partial loss in IgE binding while proteinase K treatment caused complete loss in binding to IgE. Dose-dependent inhibition in binding of rabbit anti Epi p 1 antibodies was obtained with Epi p 1, <i>Aspergillus fumigatus, Alternaria alternata, Curvularia lunata, Cladosporium herbarum<\/i> and <i>Fusarium solani<\/i> in ELISA. Rabbit antibodies to all the above five fungi recognized Epi p 1 in immunoblot, confirming that Epi p 1 shares common epitopes with the fungi tested. <i>Conclusion:<\/i> A major glycoprotein allergen of 33.5 kD was purified from EP which cross-reacts with other fungi. Hence this glycoprotein can be exploited to reduce the panel of allergen extracts used for therapy of mould allergy.<\/jats:p>","DOI":"10.1159\/000076827","type":"journal-article","created":{"date-parts":[[2004,2,20]],"date-time":"2004-02-20T09:52:42Z","timestamp":1077270762000},"page":"217-224","source":"Crossref","is-referenced-by-count":25,"title":["Purification and Characterization of a Major Cross-Reactive Allergen from <i>Epicoccum purpurascens<\/i>"],"prefix":"10.1159","volume":"133","author":[{"given":"Vandana","family":"Bisht","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Arora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.P.","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.N.","family":"Gaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susheela","family":"Sridhara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2004,4,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.113669"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000069518"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000023889"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.105220"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000023987"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:15:28Z","timestamp":1745468128000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000076827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":5,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2004,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000076827","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}