{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:03:05Z","timestamp":1778734985547,"version":"3.51.4"},"reference-count":5,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Characterization of seafood allergens is important to understand the immune response to these allergens. Moreover, a detailed comparison between atopic and nonatopic patients with adverse reactions to shrimp has never been reported. &lt;i&gt;Methods:&lt;\/i&gt; Raw and boiled shrimp extracts were analyzed by immunoblotting using sera from 9 atopic and 7 nonatopic patients with a history of adverse reactions to shrimp, and 13 control subjects. Total IgE, specific IgE and skin prick tests (SPT) to shrimp were also investigated. &lt;i&gt;Results:&lt;\/i&gt; The level of specific IgE to shrimp was higher in atopic patients than nonatopic patients (p &lt; 0.05). Symptoms, SPT results and major allergens involved were similar in atopic and nonatopic patients. The 16.5-kD protein had the highest frequency of IgE binding followed by the 40-kD protein in these patients. Other minor IgE-binding proteins were observed at the 20-, 22-, 54-, 72-, 129- and 140-kD regions. Patients who had binding to the 16.5-kD protein had either positive (25% raw\/31% cooked) or negative (13% raw\/cooked) CAP-FEIA-RAST, while patients who recognized the 40-kD protein all had positive (31% raw\/19% cooked) CAP-FEIA-RAST. All control subjects had negative immunoblots for these two proteins. &lt;i&gt;Conclusion:&lt;\/i&gt; The 16.5-kD protein was the most frequent protein identified regardless of CAP-FEIA-RAST results, while the 40-kD protein was only present in patients with positive CAP-FEIA-RAST. Therefore, 16.5-kD protein may be an important allergen that is clinically relevant in both atopic and nonatopic patients with adverse reactions to shrimp even if it is not detected by the CAP-FEIA-RAST system.<\/jats:p>","DOI":"10.1159\/000076828","type":"journal-article","created":{"date-parts":[[2004,2,20]],"date-time":"2004-02-20T09:52:42Z","timestamp":1077270762000},"page":"225-232","source":"Crossref","is-referenced-by-count":26,"title":["IgE Binding to Raw and Boiled Shrimp Proteins in Atopic and Nonatopic Patients with Adverse Reactions to Shrimp"],"prefix":"10.1159","volume":"133","author":[{"given":"Karen Thursday R.","family":"Samson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fen Hua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsushi","family":"Miura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhei","family":"Odajima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoji","family":"Iikura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Naval","family":"Rivas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Minoguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Adachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,4,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.58.9.553"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000024201"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000065172"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1001\/jama.260.10.1450"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09831-8"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76828","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/76828","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:15:29Z","timestamp":1745468129000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000076828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":5,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2004,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000076828","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}