{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:02:09Z","timestamp":1773147729890,"version":"3.50.1"},"reference-count":22,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fetal Diagn Ther"],"published-print":{"date-parts":[[2004]]},"abstract":"<i>Objective: <\/i>Prenatal diagnosis of foetal trisomies is usually performed by cytogenetic analysis. This requires lengthy laboratory procedures and it is expensive. Here, we report a retrospective study of quantitative fluorescent polymerase chain reaction (QF-PCR) for prenatal detection of trisomies 13, 18 and 21.<i> Methods:<\/i> QF-PCR was performed on a total of 447 amniotic fluids blindly analysed without any knowledge of the cytogenetic results and 43 samples with known karyotype. All samples were tested with at least 4 small tandem repeat markers specific for each chromosome 13, 18 or 21. <i>Results:<\/i> QF-PCR results on amniotic fluid were consistent with conventional cytogenetic data. QF-PCR detected 5 cases of trisomy 21, 2 cases of trisomy 18, 1 case of trisomy 13 and 1 case with Klinefelter\u2019s syndrome. <i>Conclusions:<\/i> QF-PCR has proved to be very useful in clinical settings, since it allows the detection of major numerical disorders in a few hours after sampling and thus reduces parental anxiety.<\/jats:p>","DOI":"10.1159\/000080162","type":"journal-article","created":{"date-parts":[[2004,11,9]],"date-time":"2004-11-09T07:56:35Z","timestamp":1099986995000},"page":"496-503","source":"Crossref","is-referenced-by-count":11,"title":["Prenatal Diagnosis of Common Aneuploidies Using Multiplex Quantitative Fluorescent Polymerase Chain Reaction"],"prefix":"10.1159","volume":"19","author":[{"given":"S.","family":"El Mouatassim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kuzio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ronsin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nouchy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Druard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Forestier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,11,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.57"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002%2Fbies.950170712"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0223%28199712%2917%3A13%3C1299%3A%3AAID-PD297%3E3.3.CO%3B2-8"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004390050159"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F5.12.1176"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1023%2FA%3A1022536309381"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2897%2911090-X"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F6.9.855"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2801%2906183-9"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-0705.2001.00340.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.299"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0223%28200009%2920%3A9%3C754%3A%3AAID-PD896%3E3.0.CO%3B2-N"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1017%2FS0003480098006630"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS1472-6483%2810%2962173-6"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.660"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038%2F359380a0"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.569"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F8.11.1042"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0223%28199912%2919%3A12%3C1099%3A%3AAID-PD709%3E3.0.CO%3B2-2"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093%2Fhumrep%2F15.4.929"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.655"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1006%2Fgeno.1994.1679"}],"container-title":["Fetal Diagnosis and Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80162","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80162","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:31:56Z","timestamp":1745465516000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000080162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":22,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2004,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000080162","archive":["Portico"],"relation":{},"ISSN":["1015-3837","1421-9964"],"issn-type":[{"value":"1015-3837","type":"print"},{"value":"1421-9964","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}