{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T03:45:38Z","timestamp":1760586338840,"version":"3.40.4"},"reference-count":25,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2004]]},"abstract":"Wood creosote, a mixture of phenolic compounds, inhibits enterotoxin-induced intestinal fluid secretion, suggesting that one of its constituents suppresses transepithelial Cl<sup>\u2013<\/sup> secretion from the intestinal mucosa. To identify an active constituent in wood creosote that inhibits intestinal Cl<sup>\u2013<\/sup> secretion through Cl<sup>\u2013<\/sup> channels, we first examined its effect on Cl<sup>\u2013<\/sup> secretion using a cultured cell line transfected with complementary DNA encoding a Cl<sup>\u2013<\/sup>channel and a Cl<sup>\u2013<\/sup>-sensitive fluorescent dye. We next assayed chromatographic fractions of wood creosote for the inhibitory activity on Cl<sup>\u2013<\/sup> secretion using a Ussing chamber. We found that 4,5-dimethylresorcinol, identified by gas chromatography-mass spectrometry, inhibited intestinal Cl<sup>\u2013<\/sup> secretion dose-dependently when added to a serosal, but not mucosal, surface of rat jejunum, a half-inhibitory concentration being 3.8 \u00b5g\/ml (28 \u00b5mol\/l). It was strongly suggested that this effect was due to inhibition of Cl<sup>\u2013<\/sup> channels.<\/jats:p>","DOI":"10.1159\/000080380","type":"journal-article","created":{"date-parts":[[2004,11,9]],"date-time":"2004-11-09T07:56:35Z","timestamp":1099986995000},"page":"247-253","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of Rat Intestinal Cl<sup>\u2013<\/sup> Secretion by 4,5-Dimethylresorcinol"],"prefix":"10.1159","volume":"72","author":[{"given":"Norio","family":"Ogata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,11,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM197105202842008"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM197505012921801"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.75.6.2800"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0016-5085%2800%2970358-1"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0016-5085%2899%2970502-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1023%2FA%3A1014231722696"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-7793.1997.411bb.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.2475911"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000139043"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000139111"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159%2F000139335"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000028322"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159%2F000056092"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1139%2Fm92-120"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2890%2990497-3"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1053%2Fgast.1997.v112.pm9024297"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2F0002-9343%2888%2990669-9"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.87.10.4012"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1006%2Fabio.1996.0377"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1748-1716.1951.tb00800.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.91.11.4698"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2891%2990446-6"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02354931"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1248%2Fbpb.24.623"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2FS0016-5085%2898%2970432-9"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80380","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:34:16Z","timestamp":1745465656000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000080380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2004,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000080380","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"type":"print","value":"0031-7012"},{"type":"electronic","value":"1423-0313"}],"subject":[],"published":{"date-parts":[[2004]]}}}