{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:31:23Z","timestamp":1792571483908,"version":"4.5.0"},"reference-count":27,"publisher":"S. Karger AG","issue":"4-5","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurodegener Dis"],"published-print":{"date-parts":[[2004]]},"abstract":"The neurotoxin MPTP and its active metabolite MPP<sup>+<\/sup> cause Parkinson\u2019s disease (PD)-like symptoms in vertebrates by selectively destroying dopaminergic neurons in the substantia nigra. MPTP\/MPP<sup>+<\/sup> models have been established in rodents to screen for pharmacologically active compounds. In addition to being costly and time consuming, these animal models are not suitable for large scale testings using compound libraries. We present a novel MPP<sup>+<\/sup>-based model for high-throughput screenings using the nematode <i>Caenorhabditis elegans<\/i>. Incubation of <i>C. elegans<\/i> with MPTP or its active metabolite MPP<sup>+<\/sup> resulted in strong symptomatic defects including reduced mobility and increased lethality, and is correlated with a specific degeneration of the dopaminergic neurons. The phenotypic consequences of MPTP\/MPP<sup>+<\/sup> treatments were recorded using automated hardware and software for quantification. Incubation of <i>C. elegans<\/i> with a variety of pharmacologically active components used in PD treatment reduced the MPP<sup>+<\/sup>-induced defects. Our data suggest that the <i>C. elegans<\/i> MPTP\/MPP<sup>+<\/sup> model can be used for the quantitative evaluation of anti-PD drugs.<\/jats:p>","DOI":"10.1159\/000080983","type":"journal-article","created":{"date-parts":[[2004,11,17]],"date-time":"2004-11-17T10:02:28Z","timestamp":1100685748000},"page":"175-183","source":"Crossref","is-referenced-by-count":171,"title":["<i>Caenorhabditis elegans<\/i> MPP<sup>+<\/sup> Model of Parkinson\u2019s Disease for High-Throughput Drug Screenings"],"prefix":"10.1159","volume":"1","author":[{"given":"Evelyn","family":"Braungart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Gerlach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Riederer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Baumeister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius C.","family":"Hoener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2004,11,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.042497999"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01291788"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2F0024-3205%2886%2990558-8"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.82.7.2173"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0304-3940%2888%2990705-7"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1471-4159.1993.tb13610.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2F0024-3205%2887%2990301-8"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.80.14.4546"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0169-328X%2896%2900157-X"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0306-4522%2897%2900214-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291098-2396%28199807%2929%3A3%3C225%3A%3AAID-SYN4%3E3.0.CO%3B2-8"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2885%2990482-0"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0024-3205%2885%2990067-0"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2F319056a0"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fsyn.890190107"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.282.5396.2028"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0167-7799%2801%2901925-4"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fcne.901630207"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2FS1353-8020%2800%2900055-9"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0896-6273%2800%2980944-7"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2800%2980399-2"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2F19.14.3694"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1471-4159.1997.68010058.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0896-6273%2800%2981199-X"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1159%2F000064764"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038%2F375073a0"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature01278"}],"container-title":["Neurodegenerative Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:40:12Z","timestamp":1745466012000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000080983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":27,"journal-issue":{"issue":"4-5","published-online":{"date-parts":[[2004,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000080983","archive":["Portico"],"relation":{},"ISSN":["1660-2854","1660-2862"],"issn-type":[{"value":"1660-2854","type":"print"},{"value":"1660-2862","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}