{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:54:49Z","timestamp":1790006089370,"version":"4.0.1"},"reference-count":11,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:p>Fas (APO-1\/CD95) is a transmembrane protein which mediates programmed cell death (apoptosis). Cells with a mutated &lt;i&gt;Fas&lt;\/i&gt; gene are resistant to apoptosis and thus accumulate in lesional tissues. This might provide a basis for the development of neoplasias. Genomic DNA selectively obtained from Pautrier\u2019s microabscesses in 16 cases of mycosis fungoides (MF) using a laser capture microdissection method was analyzed. &lt;i&gt;Fas&lt;\/i&gt; gene mutations were detected in 3 of 16 cases of MF (18.8%); 1 was silent and 2 were missense mutations located in exon 9. One of the 2 missense mutations involved the death domain of the &lt;i&gt;Fas&lt;\/i&gt; gene, which is essential for apoptotic signal transduction. The missense mutations resulted in the substitution of Ala with Asp at codon 220 and Ile with Thr at codon 314. Mouse T cell lymphoma cells transfected with mutant genes were resistant to apoptosis induced by the anti-Fas antibody, indicating that the missense mutations found in MF were loss-of-function mutations, thus causing the accumulation of cells in the cutaneous lesions. These findings suggest that the accumulation of lymphoid cells with &lt;i&gt;Fas&lt;\/i&gt; mutations provides, in part, a basis for the development or maintenance of MF.<\/jats:p>","DOI":"10.1159\/000080999","type":"journal-article","created":{"date-parts":[[2004,11,9]],"date-time":"2004-11-09T07:56:35Z","timestamp":1099986995000},"page":"130-134","source":"Crossref","is-referenced-by-count":19,"title":["&lt;i&gt;Fas&lt;\/i&gt; Gene Mutations in Mycosis fungoides: Analysis of Laser Capture-Microdissected Specimens from Cutaneous Lesions"],"prefix":"10.1159","volume":"67","author":[{"given":"Tomohiko","family":"Nagasawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Takakuwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Takayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Miyagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Itami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyuiki","family":"Aozasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2004,10,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2800%2981874-7"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2891%2990614-5"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1202769"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.274.5289.998"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.278.5342.1481"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2002.01794.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1205571"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.7761852"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Fng1196-255"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2895%2990013-6"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3109%2F10428199509059623"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/80999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:40:18Z","timestamp":1745466018000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000080999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2004,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000080999","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"value":"0030-2414","type":"print"},{"value":"1423-0232","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}