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Karger AG","issue":"1","license":[{"start":{"date-parts":[[2004,12,10]],"date-time":"2004-12-10T00:00:00Z","timestamp":1102636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,12,10]],"date-time":"2004-12-10T00:00:00Z","timestamp":1102636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2005]]},"abstract":"<jats:p>Treatment of normal human keratinocytes with UVC-irradiated rabbit globin mRNA 24 h before and after UVB exposure increased the survival of the human keratinocytes. We also observed that UVC-damaged mRNA reduced the formation of sunburn cells in skin models. We next tested the effects of UVC-damaged mRNA on cellular repair of DNA. DNA repair was evaluated using 2 assay methods. The first method used a damaged plasmid that is transfected back into the cell where it is repaired by the host cell repair mechanism. In these experiments, we observed that externally added UVC-damaged rabbit globin mRNA enhanced the repair of a plasmid transfected into the host keratinocyte cells. The second method used to determine the effects on DNA repair was direct immunostaining for thymidine-thymidine dimers (TT dimers) in histological sections of the skin models. Skin models were irradiated with UVB and then fixed immediately or after 24 h and stained for TT dimers. UVB irradiation immediately caused an increase in the number of stained keratinocytes in the skin. The number of stained cells decreased in skin fixed 24 h after UVB. This is due to repair of the TT dimers and their removal. Sections of skin models pretreated with UV-damaged mRNA exhibit greater removal of these TT dimers after 24 h. The above evidence suggests that damaged mRNA can trigger a host cell DNA repair pathway.<\/jats:p>","DOI":"10.1159\/000081681","type":"journal-article","created":{"date-parts":[[2004,12,17]],"date-time":"2004-12-17T21:59:19Z","timestamp":1103320759000},"page":"12-19","source":"Crossref","is-referenced-by-count":1,"title":["Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair"],"prefix":"10.1159","volume":"18","author":[{"given":"T.","family":"Mammone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Goyarts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.E.","family":"Ya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ingrassia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Marenus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Maes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2004,12,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.jidsp.5640178"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-0625.2001.100407.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.94.23.12627"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159%2F000049386"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.2307%2F3572076"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0024-3205%2865%2990046-9"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2F195614a0"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2F197090a0"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-4827%2864%2990198-3"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-4274%2898%2900007-1"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01666038"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep12499927"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.2307%2F3579402"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000256022"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038%2Fjid.1961.58"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-0625.2001.010003155.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1751-1097.1988.tb02873.x"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/81681","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/81681","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:46:45Z","timestamp":1745466405000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000081681"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,10]]},"references-count":17,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2005,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000081681","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"type":"print","value":"1660-5527"},{"type":"electronic","value":"1660-5535"}],"subject":[],"published":{"date-parts":[[2004,12,10]]}}}