{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:44:26Z","timestamp":1790837066187,"version":"4.1.0"},"reference-count":13,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T00:00:00Z","timestamp":1104105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T00:00:00Z","timestamp":1104105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Physiol"],"abstract":"<i>Background:<\/i> In this study, the mechanism of the rapid non-genomic effect of aldosterone on Na<sup>+<\/sup>\/H<sup>+<\/sup> exchanger (NHE)-mediated intracellular pH (pH<sub>i<\/sub>) recovery from an acid load in murine M-1 cortical collecting duct cells was assessed. <i>Methods:<\/i> Spectrofluorescence microscopy and Western blot analysis was carried out and NH<sub>4<\/sub>Cl was used to induce the acid load. <i>Results:<\/i> Aldosterone (10 n<i>M<\/i>) induced a rapid (<5 min) concentration-dependent increase in pH<sub>i<\/sub> recovery in M-1 cells, an effect mimicked by its precursor deoxycorticosterone (1 n<i>M<\/i>). This response was unaffected by the mineralocorticoid receptor (MR) antagonist spironolactone (10 \u00b5<i>M<\/i>) but was significantly reduced by the NHE antagonists 5\u2032-(N-ethyl- N-isopropyl)amiloride (EIPA) (20 \u00b5<i>M<\/i>) and cariporide (1 \u00b5<i>M<\/i>). The PKC inhibitor chelerythrine chloride (1 \u00b5<i>M<\/i>) significantly attenuated the aldosterone-induced increase in NHE1 activity. HBDDE (80 \u00b5<i>M<\/i>), a PKC<sub>\u03b1<\/sub> inhibitor, inhibited the rapid aldosterone effect whereas rottlerin (15 \u00b5<i>M<\/i>), a PKC<sub>\u03b4<\/sub> antagonist, did not. The glucocorticoid receptor agonists hydrocortisone (1 \u00b5<i>M<\/i>) and dexamethasone (100 n<i>M<\/i>) decreased NHE activity, whereas the synthetic mineralocorticoid fludrocortisone (1 n<i>M<\/i>) had no significant effect. MAPK inhibition using PD98059 (25 \u00b5<i>M<\/i>) significantly attenuated the rapid aldosterone effect; Western blot analysis showed that aldosterone activation of ERK 1\/2 was unaffected by pretreatment with spironolactone but was inhibited following chelerythrine chloride. <i>Conclusion:<\/i> Aldosterone causes a rapid non-genomic increase in NHE1 activity in M-1 cells via a PKC<sub>\u03b1 <\/sub>\/MAPK pathway independent of the classical MR.<\/jats:p>","DOI":"10.1159\/000081796","type":"journal-article","created":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T08:44:42Z","timestamp":1106210682000},"page":"p1-p9","source":"Crossref","is-referenced-by-count":45,"title":["Aldosterone Rapidly Activates Na<sup>+<\/sup>\/H<sup>+<\/sup> Exchange in M-1 Cortical Collecting Duct Cells via a PKC-MAPK Pathway"],"prefix":"10.1159","volume":"99","author":[{"given":"Farouk","family":"Markos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Healy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2004,12,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004240000507"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007%2Fs002329900534"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2000.00981.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.77.6.1452"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1995.2106"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.15.8763"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0039-128X%2801%2900169-6"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0039-128X%2898%2900096-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1991.148"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.274.29.20206"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0008-6363%2801%2900464-3"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-2952%2801%2900596-2"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.96.8.4686"}],"container-title":["Nephron Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/81796","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/81796","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:16:58Z","timestamp":1745464618000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000081796"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,27]]},"references-count":13,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2005,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000081796","archive":["Portico"],"relation":{},"ISSN":["1660-2137"],"issn-type":[{"value":"1660-2137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,27]]}}}