{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:10:16Z","timestamp":1782123016446,"version":"3.54.5"},"reference-count":27,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells Tissues Organs"],"published-print":{"date-parts":[[2004]]},"abstract":"Sphingosine-1-phosphate (S1P) is a lipid mediator, which affects many essential processes such as cell proliferation, differentiation and contraction in many cell types. We have previously demonstrated that the lipid mediator elicits Ca<sup>2+<\/sup> transients in a myoblastic cell line (C2C12) by interacting with its specific receptors (S1PR<sub>s<\/sub>). In the present study, we wanted to correlate the Ca<sup>2+<\/sup> response with activation of myoblastic cell contractility. C2C12 cells were first investigated for the expression and cellular organization of cytoskeletal proteins by immunoconfocal microscopy. We found that myoblasts exhibited a quite immature cytoskeleton, with filamentous actin dispersed as a web-like structure within the cytoplasm. To evaluate intracellular Ca<sup>2+<\/sup> mobilization, the cells were loaded with a fluorescent Ca<sup>2+<\/sup> indicator (Fluo-3), stimulated with S1P and simultaneously observed with differential interference contrast and fluorescence optics. Exogenous S1P-induced myoblastic cell contraction was temporally unrelated to S1P-induced intracellular Ca<sup>2+<\/sup> increase; cell contraction occurred within 5\u20138 s from stimulation, whereas intracellular Ca<sup>2+<\/sup> increase was evident only after 15\u201325 s. To support the Ca<sup>2+<\/sup> independence of myoblastic cell contraction, the cells were pretreated with a Ca<sup>2+<\/sup> chelator, BAPTA\/AM, prior to stimulation with S1P. In these experimental conditions, the myoblasts were still able to contract, whereas the S1P-induced Ca<sup>2+<\/sup> transients were completely abolished. On the contrary, when C2C12 cells were induced to differentiate into skeletal myotubes, they responded to S1P with a rapid cell contraction concurrent with an increase in the intracellular Ca<sup>2+<\/sup>. These data suggest that Ca<sup>2+<\/sup>-independent mechanism of cell contraction may be replaced by Ca<sup>2+<\/sup>-dependent ones during skeletal muscle differentiation.<\/jats:p>","DOI":"10.1159\/000082243","type":"journal-article","created":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T08:44:42Z","timestamp":1106210682000},"page":"129-138","source":"Crossref","is-referenced-by-count":5,"title":["Effects of S1P on Myoblastic Cell Contraction: Possible Involvement of Ca<sup>2+<\/sup>-Independent Mechanisms"],"prefix":"10.1159","volume":"178","author":[{"given":"D.","family":"Nosi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Vassalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Polidori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Giannini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Chellini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Paternostro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2005,1,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.34.20246"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1023%2FB%3AJURE.0000009898.02325.58"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0703516"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.cellbio.12.1.463"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M100272200"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.111.1.103"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002%2Fjcp.10366"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200112450"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1057559"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1006%2Fexcr.2000.5044"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-7793.1999.087ad.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-2999%2899%2900367-2"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.106.6.1955"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2800%2902039-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS0898-6568%2801%2900177-2"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1042%2F0264-6021%3A3620349"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038%2F365557a0"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M009422200"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1096%2Ffj.02-0836com"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1242%2Fdev.00425"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1432-1327.1999.00706.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1161%2Fhs1201.099525"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038%2F35046659"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.274.8.4626"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.11.18.2295"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002%2Fcm.970290405"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-7793.1999.0805u.x"}],"container-title":["Cells Tissues Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82243","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82243","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:21:17Z","timestamp":1745464877000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000082243"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2005,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000082243","archive":["Portico"],"relation":{},"ISSN":["1422-6405","1422-6421"],"issn-type":[{"value":"1422-6405","type":"print"},{"value":"1422-6421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}