{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:11:57Z","timestamp":1758820317070,"version":"3.40.4"},"reference-count":42,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:p>Progestins have multiple mechanisms of action in the central nervous system that are important for modulating lordosis of female rats. In the ventral tegmental area (VTA), progestins, such as the progesterone metabolite and neurosteroid 5\u03b1-pregnan-3\u03b1-ol-20-one (3\u03b1,5\u03b1-THP), regulate lordosis via actions independent of intracellular progestin receptors. We hypothesized that if, in the VTA, dopamine type 1 receptors (D&lt;sub&gt;1&lt;\/sub&gt;), G-proteins, and adenosine 3\u2032,5\u2032-monophosphate (cAMP) are downstream effectors of 3\u03b1,5\u03b1-THP\u2019s actions for lordosis, then pharmacological manipulations of these signaling molecules will produce changes in 3\u03b1,5\u03b1-THP-facilitated lordosis of estradiol (E&lt;sub&gt;2&lt;\/sub&gt;)-primed rats. VTA infusions of 3\u03b1,5\u03b1-THP (50 ng) or 3\u03b1,5\u03b1-THP and the D&lt;sub&gt;1&lt;\/sub&gt; agonist SKF38393 (100 ng) increased lordosis of ovariectomized, E&lt;sub&gt;2&lt;\/sub&gt; (10\u00a0\u00b5g)- primed rats, compared to vehicle. Both 3\u03b1,5\u03b1-THP- and 3\u03b1,5\u03b1-THP plus SKF38393-facilitated lordosis was reduced by VTA infusions of the G-protein inhibitor guanosine 5\u2032-O-(2-thiodiphosphate) (GDP-\u03b2-S; 50 \u00b5&lt;i&gt;M&lt;\/i&gt;), but not vehicle. Also, in the VTA, blocking D&lt;sub&gt;1&lt;\/sub&gt; with SCH23390 (100 ng) decreased, or increasing cAMP with 8-bromo-cAMP (200\u00a0ng) enhanced, 3\u03b1,5\u03b1-THP-facilitated lordosis of E&lt;sub&gt;2&lt;\/sub&gt;-primed rats. Notably, SCH23390\u2019s inhibitory effects on 3\u03b1,5\u03b1-THP-facilitated lordosis were reversed by 8-bromo-cAMP. Thus, in the VTA, 3\u03b1,5\u03b1-THP\u2019s actions for lordosis may involve activation of D&lt;sub&gt;1&lt;\/sub&gt; and initiation of the G-protein-mediated second messenger cAMP.<\/jats:p>","DOI":"10.1159\/000082752","type":"journal-article","created":{"date-parts":[[2004,12,17]],"date-time":"2004-12-17T21:59:19Z","timestamp":1103320759000},"page":"233-243","source":"Crossref","is-referenced-by-count":22,"title":["In the Ventral Tegmental Area, G-Proteins and cAMP Mediate the Neurosteroid 3\u03b1,5\u03b1-THP\u2019s Actions at Dopamine Type 1 Receptors for Lordosis of Rats"],"prefix":"10.1159","volume":"80","author":[{"given":"Sandra M.","family":"Petralia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl A.","family":"Frye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,1,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0018-506X%2884%2990027-8"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.143.2.436"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1095%2Fbiolreprod.103.019364"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-8993%2888%2990664-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0166-4328%2899%2900020-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1210%2Fendo-106-1-192"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2826.2004.01198.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0165-0173%2801%2900119-9"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1006%2Fhbeh.2001.1674"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000068338"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2826.2003.01047.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000125760"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.2422758"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2826.1999.00367.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.7915049"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2F0031-9384%2879%2990088-X"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.89.24.11988"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-3057%2885%2990408-3"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.287.5455.1053"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2F0031-9384%2881%2990019-6"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2FS0166-4328%2899%2900085-6"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2F0306-4530%2894%2990044-2"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2F0024-3205%2884%2990519-8"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0896-6273%2800%2980798-9"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2FS0165-0173%2897%2900057-X"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038%2F310503a0"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1037%2Fh0035941"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pbb.2004.04.014"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016%2FS0304-3959%2897%2903371-X"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-3057%2800%2900179-9"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1006%2Fhbeh.1996.0069"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1016%2F0031-9384%2871%2990120-X"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1006%2Fhbeh.1999.1562"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1016%2FS0149-7634%2805%2980229-5"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2875%2990304-0"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2826.1997.00617.x"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1210%2Fendo-119-4-1610"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1016%2F0031-9384%2889%2990016-4"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-8993%2878%2990713-8"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2878%2990130-9"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2879%2990283-8"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2880%2990308-8"}],"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:26:42Z","timestamp":1745465202000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000082752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":42,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000082752","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"type":"print","value":"0028-3835"},{"type":"electronic","value":"1423-0194"}],"subject":[],"published":{"date-parts":[[2004]]}}}