{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:50:41Z","timestamp":1777589441242,"version":"3.51.4"},"reference-count":21,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2005]]},"abstract":"The aims of the present study were to investigate the pharmacological effects induced by <i>Tityus serrulatus<\/i> venom (TsV) and its fractions and to compare with the effects induced by pure \u03b1 (TsTX-V) and \u03b2 (TsTX-I) toxins isolated from TsV on rat retractor penis muscle (RPM). TsV, fractions X, XI, XIIa, XIIb (0.01\u2013100 \u00b5g\/ml) and TsTX-V (1 nmol\/l\u201310 \u00b5mol\/l) induced concentration-dependent contractions. Prazosin and guanethidine or tetrodotoxin (TTX, 5 \u00b5mol\/l, 30 min) completely abolished these contractions, suggesting complete dependence on sympathetic nerves. TsV or fractions X, XI, XIIa, XIIb (0.01\u2013 100 \u00b5g\/ml), TsTX-I and TsTX-V (1 nmol\/l\u201310 \u00b5mol\/l) induced concentration-dependent relaxations in the precontracted RPM. TTX or N<sup>\u03c9<\/sup>-nitro-<i>L<\/i>-arginine methyl ester (<i>L<\/i>-NAME, 100 \u00b5mol\/l, 30 min) completely abolished the relaxations. Our results suggest that most of TsV-derivated toxins induce contraction and relaxation on RPM by sympathetic and NANC nitrergic nerve stimulation. Noteworthy, TsTX-I only induces relaxation on RPM suggesting that this protein selectively acts on inhibitory nerves.<\/jats:p>","DOI":"10.1159\/000082804","type":"journal-article","created":{"date-parts":[[2004,12,20]],"date-time":"2004-12-20T14:46:58Z","timestamp":1103554018000},"page":"190-198","source":"Crossref","is-referenced-by-count":3,"title":["Effects Induced by <i>Tityus serrulatus<\/i> Scorpion Venom and Its Toxins TsTX-I and TsTX-V on the Rat Isolated Retractor Penis Muscle"],"prefix":"10.1159","volume":"73","author":[{"given":"Jos\u00e9 Henrique G.G.","family":"Bomfim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio A.F.","family":"de Godoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 R.","family":"Giglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane C.","family":"Arantes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,3,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2895%2900005-7"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2874%2990238-4"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2889%2990102-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1432-1327.1999.00625.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0701623"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-4295%2800%2901047-5"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2800%2900187-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-4295%2803%2900785-4"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-4295%2803%2900462-X"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2891%2990058-Y"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2896%2900198-5"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1006%2Fabio.1976.9999"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS1056-8719%2802%2900204-6"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1104%2Fpp.54.6.813"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1440-1681.1993.tb01661.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02906497"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0896-6273%2800%2980606-6"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2898%2900206-2"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2882%2990073-3"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2892%2990874-5"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2F0742-8413%2895%2900026-K"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82804","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/82804","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:26:59Z","timestamp":1745465219000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000082804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":21,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000082804","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005]]}}}