{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:04:59Z","timestamp":1774562699852,"version":"3.50.1"},"reference-count":12,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2005]]},"abstract":"Impaired pulmonary release of nitric oxide (NO) is one of the characteristic phenotypic changes of vascular cells in pulmonary hypertension. The aim of this study was to determine nitric oxide synthase (NOS)-dependent whole body NO production in patients with primary pulmonary hypertension. NOS-dependent whole body NO production was assessed by giving an intravenous infusion of <i>L<\/i>-[<sup>15<\/sup>N]<sub>2<\/sub>-arginine (50 \u00b5mol\/min for 30 min) and measuring isotopic urinary enrichment of <sup>15<\/sup>N-nitrite and <sup>15<\/sup>N-nitrate. Four female patients with no signs of infection were recruited and compared with 6 age-matched control subjects. Mean 12-hour excretion of <sup>15<\/sup>N-nitrite and <sup>15<\/sup>N-nitrate in the total urine over 36 h was smaller in patients than in control subjects (57.2 \u00b1 27.6 vs. 229.1 \u00b1 65.2 nmol\/mmol creatinine, p< 0.01, Mann-Whitney U test, respectively). Neither mean 12-hour excretion of <sup>14<\/sup>N-nitrite and <sup>14<\/sup>N-nitrate (51.6 \u00b1 10.0 vs. 72.4 \u00b1 10.0 \u00b5mol\/mmol creatinine, p<i> = <\/i>0.3) nor glomerular filtration rates (84.5 \u00b1 15.8 vs. 129.7 \u00b1 16.0 ml\/min, p = 0.1) were different between patients and control subjects. Our results suggest that either basal NOS-dependent whole body NO production is impaired or excess NO metabolism occurs in patients with primary pulmonary hypertension.<\/jats:p>","DOI":"10.1159\/000083502","type":"journal-article","created":{"date-parts":[[2005,5,4]],"date-time":"2005-05-04T21:15:48Z","timestamp":1115241348000},"page":"133-136","source":"Crossref","is-referenced-by-count":39,"title":["Decreased Whole Body Endogenous Nitric Oxide Production in Patients with Primary Pulmonary Hypertension"],"prefix":"10.1159","volume":"42","author":[{"given":"Eric A.G.","family":"Demoncheaux","sequence":"first","affiliation":[]},{"given":"Tim W.","family":"Higenbottam","sequence":"additional","affiliation":[]},{"given":"David G.","family":"Kiely","sequence":"additional","affiliation":[]},{"given":"Ju-Ming","family":"Wong","sequence":"additional","affiliation":[]},{"given":"Simon","family":"Wharton","sequence":"additional","affiliation":[]},{"given":"Richard","family":"Varcoe","sequence":"additional","affiliation":[]},{"given":"Tom","family":"Siddons","sequence":"additional","affiliation":[]},{"given":"Alan C.","family":"Spivey","sequence":"additional","affiliation":[]},{"given":"Keith","family":"Hall","sequence":"additional","affiliation":[]},{"given":"Andy P.","family":"Gize","sequence":"additional","affiliation":[]}],"member":"127","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jacc.2004.02.029"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199507273330403"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2896%2907631-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1006%2Fabio.1996.9880"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.2165%2F00003495-199958030-00002"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200106143442404"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097%2F00005344-200104000-00016"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200301-147OC"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1749-6632.1994.tb12040.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1042%2FCS20010232"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1106830"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm954"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83502","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:33:27Z","timestamp":1745465607000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000083502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":12,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2005,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000083502","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"value":"1018-1172","type":"print"},{"value":"1423-0135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005]]}}}