{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:20Z","timestamp":1745554220723,"version":"3.40.4"},"reference-count":28,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dig Dis"],"published-print":{"date-parts":[[2004]]},"abstract":"<i>Background:<\/i> Secretory leukocyte protease inhibitor (SLPI) represents a multifunctional protein of the gastric mucosa exerting anti-microbial and anti-inflammatory effects. Recently, a local down-regulation of antral SLPI expression in <i>Helicobacter pylori <\/i>(<i>Hp)<\/i>-infected healthy volunteers was demonstrated. <i>Aim:<\/i> To analyze mucosal SLPI expression in patients with various gastroduodenal disorders. <i>Methods:<\/i> The prospective study included 90 patients with following gastroduodenal disorders diagnosed: gastric cancer (GC, n = 22), duodenal ulcer (DU, n = 17), <i>Hp<\/i>-positive dyspeptic patients (NUD, n = 31) and <i>Hp<\/i>-negative NUD (n = 20). During esophagogastroduodenoscopy, biopsies were taken each from antrum, corpus and tumor. SLPI expression was analyzed by quantitative RT-PCR and ELISA. <i>Results:<\/i> Antral SLPI levels were reduced in all <i>Hp<\/i>-infected patients (NUD, DU, GC) by about 75% (1,494\u20131,826 pg\/50 \u00b5g protein) compared to <i>Hp<\/i>-negative NUD (6,563 pg\/50 \u00b5g protein, p < 0.001, ANOVA). Tumor tissue had twofold higher SLPI levels than surrounding tumor-free gastric mucosa (3,900 vs. 1,826 pg\/50 \u00b5g protein, p = 0.013), but revealed reduced SLPI levels compared to <i>Hp<\/i>-negative NUD patients (p = 0.067). No differences were found between SLPI expression of intestinal and diffuse GC. SLPI transcript levels were unchanged throughout all groups and locations implying that transcriptional regulation of SLPI is not involved. <i>Conclusion:<\/i> Local down-regulation of SLPI in antral mucosa is a general phenomenon of <i>Hp<\/i>-related diseases.<\/jats:p>","DOI":"10.1159\/000083604","type":"journal-article","created":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T08:14:07Z","timestamp":1112343247000},"page":"390-395","source":"Crossref","is-referenced-by-count":4,"title":["Down-Regulation of Secretory Leukocyte Protease Inhibitor Expression in Gastric Mucosa Is a General Phenomenon in <i>Helicobacter pylori<\/i>-Related Gastroduodenal Diseases"],"prefix":"10.1159","volume":"22","author":[{"given":"Thomas","family":"Wex","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Sokic-Milutinovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Todorovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milos","family":"Bjelovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomica","family":"Milosavljevic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Predrag","family":"Pesko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Malfertheiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,3,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-5378.7.s1.6.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097%2F00042737-200012070-00013"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.tim.2003.11.005"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.93.3.1259"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1053%2Fgast.2000.8540"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1067147"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1080%2F003655201750422602"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2F21.11.2091"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2559.2003.01731.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1053%2Fj.gastro.2003.06.007"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1128%2FIAI.72.4.2383-2385.2004"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2F80489"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2802%2901141-8"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M203710200"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.190.4.535"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1186%2Frr18"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-0691.2003.00582.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1097%2F00003246-200005000-00003"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.45.2008.ii37"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097%2F00000478-199610000-00001"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1080%2F00365520310008403"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1515%2FCCLM.2003.159"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1525-1438.2001.01062.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0065-2571%2898%2900020-X"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.1037154100"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2036.17.s2.10.x"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10120-003-0265-0"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.2174%2F1381612043383566"}],"container-title":["Digestive Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83604","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:34:16Z","timestamp":1745465656000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000083604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":28,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000083604","archive":["Portico"],"relation":{},"ISSN":["0257-2753","1421-9875"],"issn-type":[{"type":"print","value":"0257-2753"},{"type":"electronic","value":"1421-9875"}],"subject":[],"published":{"date-parts":[[2004]]}}}