{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:10:24Z","timestamp":1745554224281,"version":"3.40.4"},"reference-count":35,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2005]]},"abstract":"<i>Background:<\/i> Glucocorticoids can boost some <i>Malassezia<\/i>-driven dermatoses. However, both antifungals and topical corticosteroids improve lesions of seborrheic dermatitis. <i>Objective:<\/i> To revisit the topical activity of the antifungal ketoconazole and the corticosteroid desonide on <i>Malassezia<\/i> growth on human stratum corneum. <i>Material and Methods:<\/i> The computer-assisted corneofungimetry bioassay was used to compare the growth of <i>M. furfur, M. globosa<\/i> and <i>M. restricta <\/i>on human stratum corneum coated with olive oil. Four blinded gel formulations were tested. They contained either 2% ketoconazole, 0.05% desonide or a combination of 2% ketoconazole and 0.05% desonide; one gel was unmedicated. Untreated stratum corneum and specimens coated with a 2% ketoconazole cream were used as negative and positive comparators, respectively. A total of 45 samples (15 <i>M. furfur<\/i>, 15 <i>M. globosa<\/i>, and 15 <i>M. restricta)<\/i> were used for each test formulation in this randomized, double-blind study. <i>Results:<\/i> The 2% ketoconazole gel and cream and the combination of 2% ketoconazole and 0.05% desonide formulation abated similarly and significantly the <i>M. furfur<\/i>, <i>M. globosa <\/i>and<i> M. restricta<\/i> growth. The 3 species were similarly sensitive to these formulations. By contrast, no significant inhibitory effect was yielded by the 0.05% desonide gel and the vehicle. <i>Conclusion:<\/i> The presence of 0.05% desonide does not impair or improve the <i>Malassezia<\/i> susceptibility to 2% ketoconazole when growing on lipid-enriched human stratum corneum.<\/jats:p>","DOI":"10.1159\/000083710","type":"journal-article","created":{"date-parts":[[2005,3,16]],"date-time":"2005-03-16T09:57:17Z","timestamp":1110967037000},"page":"98-102","source":"Crossref","is-referenced-by-count":4,"title":["Corneofungimetry Bioassay on <i>Malassezia<\/i> spp. under Ketoconazole and Desonide Influences"],"prefix":"10.1159","volume":"18","author":[{"given":"C.","family":"Pi\u00e9rard-Franchimont","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Vroome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Cauwenbergh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.E.","family":"Pi\u00e9rard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,3,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2230.1997.d01-231.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000068903"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1468-3083.2004.00693.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159%2F000076478"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2133.1986.tb04878.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0190-9622%2890%2970191-J"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2F0926-9959%2894%2900038-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2165%2F00128071-200001020-00001"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2133.2001.04082.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00873693"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00399623"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2133.2000.03294.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1080%2F714030958"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.40.9.3350-3357.2002"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1439-0507.1995.tb00070.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1439-0507.1996.tb00145.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.44.2.467-469.2000"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-4362.1991.tb04793.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159%2F000018337"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0926-9959%2897%2900165-7"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-4362.1999.00783.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.1996.d01-281.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2002.00132.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1159%2F000069968"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00373427"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007%2Fs002280050193"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1159%2F000063382"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2230.1989.tb02603.x"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1159%2F000211137"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1080%2F02681219580000271"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1159%2F000057885"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep13071328"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1080%2F02681219480000051"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2001.01321.x"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep12687967"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83710","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:36:02Z","timestamp":1745465762000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000083710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2005,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000083710","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"type":"print","value":"1660-5527"},{"type":"electronic","value":"1660-5535"}],"subject":[],"published":{"date-parts":[[2005]]}}}