{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:34:28Z","timestamp":1760204068953,"version":"3.40.4"},"reference-count":31,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2005]]},"abstract":"<i>Background:<\/i> Interleukin (IL)-4 plays a prominent role in immune response. Mercuric compounds upregulate IL-4 expression in animal tissues, and this upregulation plays a role in mercuric-mediated immunomodulation. Mercuric ions-mediated IL-4 expression was observed in vitro in T lymphocytes and mast cells. In the present study, we investigated molecular mechanisms responsible for this effect of mercuric ions in mast cells. <i>Methods:<\/i> C1.MC\/C57.1 mouse mast cells were exposed in vitro to increasing concentrations of Hg<sup>2+<\/sup> in the absence or presence of the specific c-Jun N-terminal kinase (JNK) inhibitor SP600125. The level of phosphorylated c-Jun in mast cells was determined by Western blotting, JNK activity assessed with in vitro kinase assay and the amount of secreted IL-4 determined by ELISA. <i>Results:<\/i> We observed that Hg<sup>2+<\/sup> upregulated c-Jun phosphorylation on Ser 73 at concentrations which overlapped concentrations mediating IL-4 secretion. Phosphorylation of c-Jun in mast cells was associated with an increase in JNK activity. The specific JNK inhibitor SP600125 abolished both mercuric-induced c-Jun phosphorylation and IL-4 secretion in mast cells. <i>Conclusions:<\/i> These observations are consistent with the hypothesis that JNK is one of the signaling proteins mediating the effect of Hg<sup>2+<\/sup> on IL-4 expression in mast cells and is engaged in environmentally mediated immunomodulation.<\/jats:p>","DOI":"10.1159\/000083892","type":"journal-article","created":{"date-parts":[[2005,2,11]],"date-time":"2005-02-11T05:58:20Z","timestamp":1108101500000},"page":"181-190","source":"Crossref","is-referenced-by-count":11,"title":["c-Jun N-Terminal Kinase Is Involved in Mercuric Ions-Mediated Interleukin-4 Secretion in Mast Cells"],"prefix":"10.1159","volume":"136","author":[{"given":"Aurelia","family":"Walczak-Drzewiecka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janina","family":"Wycz\u00f3\u0142kowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaros\u0142aw","family":"Dastych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,2,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2307%2F3434338"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1053%2Fajcd.2000.7186"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.2307%2F3434336"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.1830251029"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-3083.1995.tb03563.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2249.1998.00704.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970186-7"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.1830250822"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.272.51.32411"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F1074-7613%2895%2990028-4"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2F19.17.4783"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2F353670a0"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.7.11.2135"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.8.24.2996"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS0955-0674%2898%2980143-9"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS1357-2725%2801%2900093-0"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1006%2Ftaap.1999.8772"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093%2Ftoxsci%2F53.2.361"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-4644%2820000901%2978%3A3%3C500%3A%3AAID-JCB14%3E3.0.CO%3B2-P"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.22.12762"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2F19.20.5387"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2FS0076-6879%2895%2955037-2"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2894%2990380-8"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.90.20.9707"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-3083.1996.d01-337.x"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.251194298"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200112466"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2887%2990339-4"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1038%2F339064a0"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.176.5.1381"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1159%2F000236482"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83892","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/83892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:37:21Z","timestamp":1745465841000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000083892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2005,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000083892","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2005]]}}}