{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:52:04Z","timestamp":1761580324471,"version":"3.40.4"},"reference-count":38,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2005]]},"abstract":"<i>Objective:<\/i> Mycosis fungoides (MF) is the most frequent form of cutaneous T cell lymphoma (CTCL). Human T cell leukemia virus type 1 (HTLV-1) involvement in MF progression is a matter of debate. The goal of the investigation was to search for HTLV-1 markers in a group of MF patients from a nonendemic area to HTLV-1. <i>Materials and Methods:<\/i> Fifty MF patients and 60 healthy donors from Moscow and the Moscow region were examined for HTLV-1 markers by Western blot, PCR, nested PCR, PCR\/Southern hybridization, TaqMan real-time PCR and sequencing. <i>Results:<\/i> Plasma samples from MF patients were repeatedly negative for antibodies to HTLV-1 structural proteins. HTLV-1 <i>tax<\/i>-related sequences (corresponding to the second exon) were found in blood from 20 of 50 MF patients and in 3 of 5 saliva specimens. Three of 8 sequenced <i>tax-<\/i>like amplimers were identical and 5 of 8 contained 1\u20132 substitutions. <i>tax<\/i> transcripts and antibodies to p40<sup>tax<\/sup> were detected in some \u2018PCR-<i>tax\u2019<\/i>-positive MF patients. Defective HTLV-1 genomes were demonstrated in 2 of 50 MF patients. Phylogenetic analysis of the defective genome 5\u2032-LTR sequence revealed a relationship with HTLV-1a sequences from the transcontinental subgroup of HTLV-1. <i>Conclusions:<\/i> HTLV-1 <i>tax-<\/i>like sequences were revealed in blood and for the first time in saliva from MF patients living in an HTLV-1 nonendemic region. Expression of <i>tax-<\/i>like sequences was confirmed by both reverse transcription PCR and Western blot.<\/jats:p>","DOI":"10.1159\/000086063","type":"journal-article","created":{"date-parts":[[2005,7,15]],"date-time":"2005-07-15T07:33:48Z","timestamp":1121412828000},"page":"362-371","source":"Crossref","is-referenced-by-count":15,"title":["Mycosis fungoides in European Russia: No Antibodies to Human T Cell Leukemia Virus Type I Structural Proteins, but Virus-Like Sequences in Blood and Saliva"],"prefix":"10.1159","volume":"48","author":[{"given":"Vladimir A.","family":"Morozov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Syrtsev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Ellerbrok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena V.","family":"Nikolaeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei S.","family":"Bavykin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Pauli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,7,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.77.12.7415"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.78.10.6476"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2885%2992734-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2886%2991298-5"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9394%2802%2901521-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.267.2.236b"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01718595"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002%2Fjmv.1890390315"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-8652%28199610%2953%3A2%3C133%3A%3AAID-AJH13%3E3.0.CO%3B2-G"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-9071%28199705%2952%3A1%3C1%3A%3AAID-JMV1%3E3.0.CO%3B2-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1089%2Faid.1991.7.923"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F21.16.3799"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1128%2FJVI.74.9.3933-3940.2000"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1006%2Fviro.1999.9922"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMra032810"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.immunol.19.1.475"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI117697"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128%2FCDLI.7.2.274-278.2000"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1749-6632.2001.tb03713.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1857968"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.8451645"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1537-2995.1999.39080904.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1128%2FCDLI.10.4.715-717.2003"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS1079-9796%2803%2900124-4"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2F0145-2126%2893%2990093-Z"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1006%2Fviro.1998.9578"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1101%2Fgr.6.10.986"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01731581"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.2307%2F2408678"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-9071%28199806%2955%3A2%3C152%3A%3AAID-JMV12%3E3.0.CO%3B2-K"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0215%2819980812%2977%3A4%3C488%3A%3AAID-IJC2%3E3.0.CO%3B2-Q"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1089%2Faid.1998.14.1199"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0215%2820000801%2987%3A3%3C379%3A%3AAID-IJC11%3E3.0.CO%3B2-O"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.94.12.6403"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2133.1999.02919.x"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2133.1991.tb00434.x"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.180.5.1973"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2141.1997.3213138.x"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86063","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:08:19Z","timestamp":1745464099000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000086063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":38,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2005,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000086063","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"type":"print","value":"0300-5526"},{"type":"electronic","value":"1423-0100"}],"subject":[],"published":{"date-parts":[[2005]]}}}