{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:24:51Z","timestamp":1757543091559,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2005]]},"abstract":"<i>Objective:<\/i> To evaluate the potential value of quantitative fluorescent polymerase chain reaction (QF-PCR) in the detection of chromosome abnormalities in ectopic pregnancies. <i>Methods:<\/i> Seventy chorionic villi samples of ectopic pregnancies were studied by QF-PCR. Primers for chromosomes 16, 21, X and Y in chorionic villi were evaluated. Fluorescence in situ hybridization (FISH) was performed when results of QF-PCR showed aneuploidy, in case of unexplicable QF-PCR peaks, and in 10 cases with normal QF-PCR results. <i>Results:<\/i> QF-PCR produced a result for chromosomes X and Y in 66 cases (94%), for chromosome 16 in 62 cases (89%) and for chromosome 21 in 55 cases (79%). Overall, QF-PCR produced a result for the chromosomes tested in 54 ectopic pregnancy cases (77%). Fifty-two of these results were normal disomic (96%) and two were abnormal, one trisomy 16 (2%) and one triploidy (2%). In 16 cases (23%) no definite QF-PCR results could be obtained for all chromosomes, 11 due to amplification failure, and 5 due to unexplicable QF-PCR peaks. In 10 cases with normal QF-PCR results, disomy was confirmed by FISH. The trisomy 16 was also confirmed by FISH. Furthermore, a result was obtained with FISH in 5 of the cases without definite QF-PCR results. <i>Conclusion:<\/i>Although QF-PCR can establish the chromosomal status in ectopic pregnancies for chromosomes 16, 21, X and Y in the majority of cases, the technical failure rate is still considerable and does not improve results when compared to cytogenetic techniques.<\/jats:p>","DOI":"10.1159\/000086131","type":"journal-article","created":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T07:59:50Z","timestamp":1117612790000},"page":"139-144","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Chromosome Abnormalities by Quantitative Fluorescent PCR in Ectopic Pregnancies"],"prefix":"10.1159","volume":"60","author":[{"given":"Mariette","family":"Goddijn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marja","family":"van Stralen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heleen","family":"Schuring-Blom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bert","family":"Redeker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liesbeth","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sjoerd","family":"Repping","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nico","family":"Leschot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fulco","family":"van der Veen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,10,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093%2Faje%2Fkwf190"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.1970130506"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS1071-5576%2898%2900027-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0015-0282%2800%2901593-4"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136%2Fjmg.35.2.126"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0223%28199807%2918%3A7%3C669%3A%3AAID-PD324%3E3.3.CO%3B2-G"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-0705.2001.00340.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9378%2897%2970292-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fpd.301"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1530%2Frep.0.1260279"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093%2Fhumrep%2F17.5.1166"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1017%2FS0003480098006630"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F7.10.1001"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-1809.2001.6550421.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038%2Fng754"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86131","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:09:20Z","timestamp":1745464160000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000086131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2005,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000086131","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"type":"print","value":"0378-7346"},{"type":"electronic","value":"1423-002X"}],"subject":[],"published":{"date-parts":[[2005]]}}}