{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:09:18Z","timestamp":1745554158161,"version":"3.40.4"},"reference-count":14,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T00:00:00Z","timestamp":1130284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T00:00:00Z","timestamp":1130284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Physiol"],"abstract":"<i>Background:<\/i><i>L<\/i>-Arginine (<i>L<\/i>-Arg), a substrate of nitric oxide synthases, improves renal function in ischemic acute renal failure (iARF). We evaluated whether <i>L<\/i>-Arg improves renal morphology and cell survival in the course of iARF. <i>Methods and Results:<\/i> iARF was induced in rats by bilateral clamping of renal arteries for 45 min. <i>L-<\/i>Arg was applied intraperitoneally during clamping, and orally during 14 days of follow-up. Morphology and cell survival of renal cortical and medullar tissue was analyzed on days 1, 3, 7, and 14 of follow-up, using toluidine blue staining and immunohistochemistry of perfusion-fixated tissue, and Western blot analysis of tissue homogenate. Renal tubular injury showed typical features of necrosis and was most severe on days 1 and 3 after clamping, predominantly in S3 segments, with almost complete recovery by day 14. Enhanced medullar monocyte infiltration, determined by ED-1 expression as well as by immunohistochemistry, and enhanced expression of proliferating cell nuclear antigen (PCNA), indicative of proliferation and regeneration, accompanied these morphological changes. Compared to controls, <i>L<\/i>-Arg had no impact on renal morphology, ED-1, and PCNA expression. Furthermore, expression of markers of apoptosis Bcl-2, Bax, and cleaved caspase-3 was only slightly increased in iARF rats, compared to sham-operated animals, and was also not influenced by <i>L<\/i>-Arg. <i>Conclusion:<\/i> Despite its repeatedly reported positive impact on renal function as also shown in our model, <i>L<\/i>-Arg does not alter cell death and proliferation in the course of iARF in our model. Thus, different mechanisms have to be considered, in particular improved intrarenal hemodynamics.<\/jats:p>","DOI":"10.1159\/000086647","type":"journal-article","created":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T14:18:26Z","timestamp":1120227506000},"page":"p39-p50","source":"Crossref","is-referenced-by-count":13,"title":["<i>L<\/i>-Arginine Does Not Affect Renal Morphology and Cell Survival in Ischemic Acute Renal Failure in Rats"],"prefix":"10.1159","volume":"101","author":[{"given":"Ulrike","family":"Raff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stepan","family":"Gambaryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Seibold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Reber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Vornberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Freund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lothar","family":"Schramm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Wanner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Galle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,10,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.89.24.11993"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2002.00268.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2003.00063.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2F14.6.1398"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1994.176"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2001.059002515.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1006%2Fabio.1976.9999"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1978.87"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097%2F00024382-199709000-00006"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1136%2Fmp.51.4.204"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2F15.3.318"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2002.t01-1-00622.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1997.183"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ASN.0000033275.17169.67"}],"container-title":["Nephron Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86647","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86647","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:13:38Z","timestamp":1745464418000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000086647"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,26]]},"references-count":14,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2005,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000086647","archive":["Portico"],"relation":{},"ISSN":["1660-2137"],"issn-type":[{"type":"electronic","value":"1660-2137"}],"subject":[],"published":{"date-parts":[[2005,10,26]]}}}