{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:09:18Z","timestamp":1745554158723,"version":"3.40.4"},"reference-count":14,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2005]]},"abstract":"<jats:p>&lt;i&gt;Purpose:&lt;\/i&gt; Production of the proinflammatory cytokine interleukin (IL) 1\u03b1, which is stored in keratinocytes (KCs) and is multifunctional, may be enhanced by glucocorticoids. A study was made to confirm whether immunosuppressants such as the macrocyclic lactone FK506 (tacrolimus) possibly serve to modulate glucocorticoid- stimulated IL-1\u03b1 production from chemically treated KCs.&lt;i&gt; Methods:&lt;\/i&gt; Usingcultured human KCs, the effects of FK506 on the production of IL-1\u03b1 treated with chemicals (trinitrobenzene sulfonic acid sodium salt, TNBS) plus hydrocortisone were analyzed by ELISA and mRNA expression of IL-1\u03b1 using RT-PCR. &lt;i&gt;Results:&lt;\/i&gt; Intracellular IL-1\u03b1 in TNBS-treated human KCs was increased with a low concentration of 10&lt;sup&gt;\u201310&lt;\/sup&gt;&lt;i&gt;M&lt;\/i&gt; of hydrocortisone. Further FK506 application inhibited significantly this increased production.The mRNA expression of IL-1\u03b1 at various concentrations of chemicals was not remarkably different. &lt;i&gt;Conclusions:&lt;\/i&gt; FK506may control the increase in IL-1\u03b1 with glucocorticoid in KCs, suggesting FK506 to suppress harmful effects of glucocorticoids such as steroid rosacea.<\/jats:p>","DOI":"10.1159\/000086670","type":"journal-article","created":{"date-parts":[[2005,7,6]],"date-time":"2005-07-06T08:40:24Z","timestamp":1120639224000},"page":"241-246","source":"Crossref","is-referenced-by-count":4,"title":["FK506 (Tacrolimus) Inhibition of Intracellular Production and Enhancement of Interleukin 1\u03b1 through Glucocorticoid Application to Chemically Treated Human Keratinocytes"],"prefix":"10.1159","volume":"18","author":[{"given":"Y.","family":"Horiuchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.-J.","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,8,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep12316087"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.164.6.2095"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2000.00101.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159%2F000051374"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaad.2004.03.030"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0167-5699%2890%2990011-W"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jdermsci.2004.06.004"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1067%2Fmjd.2001.114739"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.10215"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2F386728a0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.2174%2F0929867043455576"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0968-0896%2896%2900229-5"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0231020100"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.273.20.12378"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86670","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86670","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:13:47Z","timestamp":1745464427000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000086670"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":14,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2005,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000086670","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"type":"print","value":"1660-5527"},{"type":"electronic","value":"1660-5535"}],"subject":[],"published":{"date-parts":[[2005]]}}}