{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:09:28Z","timestamp":1745554168706,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"published-print":{"date-parts":[[2005]]},"abstract":"<i>Objective:<\/i> To determine the prevalence of extended-spectrum \u03b2-lactamase (ESBL)-producing members of the Enterobacteriaceae using VITEK 2 and E test systems. <i>Materials and Methods:<\/i> A total of 3,592 consecutive gram-negative isolates (single isolate per patient) of the family of Enterobacteriaceae and <i>Pseudomonas<\/i> adjudged to be clinically relevant to the patient\u2019s infection were studied for ESBL production over a period of 1 year at Mubarak Al-Kabeer Hospital, Kuwait. Two methods were used: the automated VITEK 2 system and E test ESBL, a manually manipulated plastic strip containing various gradients of \u03b2-lactam antibiotics. These tests and interpretative criteria for the results were performed according to the manufacturer\u2019s instructions. <i>Results:<\/i> Of the 3,592 bacterial isolates, 264 (7.5%) and 185 (5.2%) were positive for ESBL production by the VITEK 2 and E test, respectively. All the ESBL-producing <i>Pseudomonas aeruginosa<\/i> identified by VITEK 2 gave indeterminate results by E test. Prevalent ESBL producers, identified by the VITEK 2 versus E test, respectively, were: <i>Citrobacter<\/i> spp. (15 vs. 3.2%), <i>K. pneumoniae<\/i> (12.2 vs. 11.4%), <i>Enterobacter<\/i> spp. (12 vs. 3%), <i>E. coli<\/i> (6.5 vs. 5.6%), <i>P. aeruginosa<\/i> (6.5 vs. 0%) and <i>Morganella<\/i> spp. (2 vs. 1%). The most common infection associated with ESBL-producing pathogens was urinary tract infection (68.2%), followed by wound infection (14.4%) and bloodstream infection (6.1%). <i>Conclusion:<\/i> The result of this study showed a relatively high prevalence of clinically significant ESBL producers among the Enterobacteriaceae and <i>Pseudomonas<\/i> spp. at our teaching hospital. The VITEK 2 identified a higher prevalence of ESBL strains than the E test.<\/jats:p>","DOI":"10.1159\/000086930","type":"journal-article","created":{"date-parts":[[2005,8,15]],"date-time":"2005-08-15T10:29:59Z","timestamp":1124101799000},"page":"325-331","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of Extended-Spectrum Beta-Lactamases in Enterobacteriaceae, <i>Pseudomonas <\/i>and <i>Stenotrophomonas<\/i> as Determined by the VITEK 2 and E Test Systems in a Kuwait Teaching Hospital"],"prefix":"10.1159","volume":"14","author":[{"given":"Wafaa","family":"Jamal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.O.","family":"Rotimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Khodakhast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolla","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleyamma","family":"Pazhoor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghyada","family":"Al Hashim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,8,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1086%2F344654"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F42.2.128"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.281.6.517"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1086%2F319757"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.39.6.2206-2212.2001"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0732-8893%2801%2900286-3"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.39.7.2379-2385.2001"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01690090"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1089%2F10766290152773383"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2805%2979231-X"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F45.6.895"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.44.11.3177-3179.2000"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.46.1.196-202.2002"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007%2Fs150100050041"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F44.4.489"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.40.10.3703-3711.2002"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F37.1.203"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1198-743x.2001.00330.x"}],"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86930","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/86930","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:17:09Z","timestamp":1745464629000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000086930"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":18,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2005,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000086930","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"type":"print","value":"1011-7571"},{"type":"electronic","value":"1423-0151"}],"subject":[],"published":{"date-parts":[[2005]]}}}